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Example sentences for: dynal

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mRNA selection from 100 μg total RNA using oligo-d(T) 25 Dynabeads (Dynal) and following the manufacturer's directions yielded 2-3 μg poly(A) +-enriched RNA in 20 μl 10 mM Tris-HCl, pH 7.5.
After 72 hr activation, PBMC were washed and replaced in culture without stimulation for 36-40 hr prior to restimulation with 1 nM IL-2 [ 16 ] . To purify T lymphocytes, 72 hr OKT3-activated PBMC were depleted of CD56, and positively selected for CD2, using antibody-conjugated magnetic beads (anti-CD56 Polysciences Inc, anti-CD2 Dynal) according to manufacturer's instructions.
10 TCR × hCARΔcyt double transgenic mice by positive sorting using anti-CD4 magnetic beads (Dynal AS, Oslo, Norway).
After addition of 10 ul of fetal calf serum and 10 ul of antibody "mix" (T cell negative isolation kit from Dynal Inc), the cells were incubated 10 minutes at 4°, washed in PBS, and suspended in 900 ul PBS with 100 ul of "depletion" beads (Dynal).
Immunoprecipitation of all receptor types was performed using magnetic Dynabeads (Dynal Bioscience, Lake Success, NY) coated with anti-mouse or anti-rabbit IgG and used according to the manufacturer's instructions.