Words similar to duplex
Example sentences for: duplex
How can you use “duplex” in a sentence? Here are some example sentences to help you improve your vocabulary:
The strong correlation in the array and solution data in Figure 1Bdemonstrate that the mismatches at the 3' end of the oligodeoxyribonucleotide did not destabilize the duplex significantly, explaining the lack of a decrease in the signal intensity of these mismatches compared to the perfect match.
Modified nucleotides appear to drive the equilibrium (particularly for low copy transcripts binding with their targets) towards duplex formation.
Probe density may result in steric effects [ 17 ] and could affect the efficiency of duplex formation and kinetics of target capture [ 18 ] .
Earlier studies have shown that DAP, in DNA fragments, can widen the minor groove, as detected by reactivity towards uranyl nitrate or susceptibility to Dnase cleavage [ 29 ] , and can relieve compressions in the minor groove associated with A-tracts [ 30 ] . In contrast, NMR and molecular modeling studies of an oligonucleotide duplex containing MeC have demonstrated that, while this duplex still has a B-DNA conformation, there are differences in the structural parameters and thermal stability relative to the unmodified duplex [ 31 ] . An earlier study also found, in two closely related octanucleotide duplexes, that although the methylated duplexes retained their B-DNA conformation, different structural and thermal stability effects were seen [ 32 ] . Although these studies have been carried out with DNA-DNA and not DNA-RNA duplexes, it is possible that MeC substitution may have different structural consequences than DAP substitution.
The duplex or heteroduplex substrates were prepared by mixing the labeled top strand with 4 fold excess of the unlabeled bottom strand, heating at 80°C for three minutes, cooling to room temperature and purified by electrophoresis in a 20% non-denaturing polyacrylamide gel with TBE buffer.
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