Example sentences for: double-immunofluorescence

How can you use “double-immunofluorescence” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • In order to establish if the MIF stained cells were neurons or glia, we employed double-immunofluorescence with MIF and GFAP.

  • Sections from the MPG, the IMG and the sympathetic chain were processed for double-immunofluorescence in order to visualize both MIF and tyrosine hydroxylase, as follows: Sections were exposed to 3% normal serum (in 0.01 M phosphate buffer-saline, 0.1 % Tx-100; 30 min) followed by incubation with anti-MIF (1:200) and anti-tyrosine hydroxylase (sheep polyclonal antibody; 1:1000; Pel-Freez; Rogers, AR) overnight at 4°C.

  • Some spinal cord sections were also processed for MIF and GFAP double-immunofluorescence as follows: Sections were incubated with anti-MIF (1:200) and anti-GFAP (mouse monoclonal; 1:1000; Chemicon).

  • Control experiments aimed to demonstrate the specificity of the staining with the MIF antibody and to rule out cross-reactivity of secondary antisera included: 1) preabsorption controls: incubation of the MIF antibody with the peptide used to produce the antibody; 2) omission of the primary antibody; 3) omission of either one of the primary antisera used for double-immunofluorescence.

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