Words similar to dotmac

Example sentences for: dotmac

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an alternative method, we extracted cells also with DOTMAC and discovered that fixation and extraction with 1.0 % PFA/0.
The monoclonal antibody reagent specific for β-actin worked only in TCA or DOTMAC/PFA-treated cells (Figure 3Oand 3S).
The DOTMAC/PFA method thus should be considered the method of choice for the preservation and immunostaining of microextensions or when cells fixed by other methods cannot be stained with certain antisera.
Although MTSB, which is essentially similar to PHEM, PEM, or MTPB, was shown to give the best preservation of the cytoskeleton, no morphological differences were seen between these buffers and PBS(+), when the cells were extracted with DOTMAC (data not shown).
5 % DOTMAC in PBS(+) on ice for 5 min, followed by fixation with 1.0% PFA in PBS on ice for 20 min (DOTMAC/PFA method), preserved not only micropodia (white arrow, Figure 2, Eand 2F), but also microvilli (black arrow, Figure 2Eand 2F), and ultramicroextensions (white arrowhead, Figure 2F).