Words similar to dotmac
Example sentences for: dotmac
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This effect is relatively specific since DOTMAC disrupted microtubules and they appeared as amorphous filaments (Figure 8D).
DOTMAC/PFA was superior to TCA and the standard Triton X-100 permeabilization procedure to preserve microextensions and for moesin staining (Figure 4, G, K, and 4N,O,P).
Although MTSB, which is essentially similar to PHEM, PEM, or MTPB, was shown to give the best preservation of the cytoskeleton, no morphological differences were seen between these buffers and PBS(+), when the cells were extracted with DOTMAC (data not shown).
However, no morphological difference between PFA and formaldehyde was apparent when cells were extracted with DOTMAC (data not shown).
While this may be true for some proteins, the postulated cytoskeletal association of threonine558-phosphorylated moesin was maintained only when platelets were extracted with DOTMAC, but not with Triton X-100 [ 19].