Words similar to dotmac

Example sentences for: dotmac

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Although extraction can be done before, during, or after fixation, we used simultaneous extraction and fixation, because (i) cytoskeletal molecules may be rearranged or altered during fixation, as irreversible crosslinking of polypeptides is not complete even after 15 min incubation with 4% PFA at 37°C; (ii) although actin was more difficult to extract, moesin and other polypeptides were removed already with 0.5% DOTMAC in the absence of PFA.
Evaluation of DOTMAC/PFA method by fluorescent microscopy
We have previously shown that purified threonine558-phosphomoesin selectively co-sedimented with actin filaments in the presence of DOTMAC or PIPs, but excess Triton X-100 disrupted the interaction of moesin with PIPs [ 19].
On the contrary, bundling of actin filaments by DOTMAC may have stabilized these actin-containing structures.
This effect is relatively specific since DOTMAC disrupted microtubules and they appeared as amorphous filaments (Figure 8D).