Example sentences for: dodecyl

How can you use “dodecyl” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • For direct comparisons of protein expression between drug-treated and untreated cells, equal amounts of protein were loaded in adjacent lanes on a single polyacrylamide gel, separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions and transferred to nitrocellulose membranes by the semi-dry transfer method.

  • Polyacrylamide gel electrophoresis and Western blot analyses were performed as described [ 13 23 ] . Briefly, samples were electrophoresed on 10% resolving sodium dodecyl sulphate-polyacrylamide gels (SDS/PAGE) according to the method of Laemmli [ 35 ] . Proteins were then electrotransferred onto nitrocellulose membranes and the membranes were incubated in TBST buffer containing 5% nonfat dry milk for 1 hour to block nonspecific protein binding.

  • SDS, sodium dodecyl sulfate

  • Approximately 200 ng of the pre-amplified AFLP fragments (length range from 100 bp - 600 bp) were added to a single reaction mixture containing 4.2 × SSC (Saline-Sodium Citrate, pH 7.0), 0.07% SDS (sodium dodecyl sulfate), and 10 pmol biotinylated probe.

  • The TCA-precipitated proteins were pelleted by centrifugation (20,800 × g at 4°C) for 20 min and resuspended in 2X sodium dodecyl sulfate (SDS) sample buffer [ 34 ] . Protein extracts for affinity-purification were prepared by disintegration with a French press.


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