Words similar to dodecyl
Example sentences for: dodecyl
How can you use “dodecyl” in a sentence? Here are some example sentences to help you improve your vocabulary:
SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis
The lysates were centrifuged at 4000 × g for 15 minutes, then aliquoted and stored at -20°C prior to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and western analysis as previously described [ 12 ] . Protein concentrations of the cell lysates were determined using the Bio-Rad Protein Assay (Hercules, CA), and sample volumes adjusted so that 5-10 μg of total protein were loaded into wells of a discontinuous (12% separating, 4% stacking) polyacrylamide gel.
Purified PCR products were dried down and resuspended in 80 ul 3X SSC (standard saline citrate- 1X SSC = 150 mM NaCl, 15 mM sodium citrate) with 0.01% sodium dodecyl sulfate (SDS).
Whole cell lysate (50 μg) was loaded onto a 6% sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel, transferred to nitrocellulose, and hybridized with an anti-BRCA1 (Ab-1; Oncogene Research, Cambridge, MA, USA) antibody as previously described [ 10].
Sodium dodecyl sulfate polyacrylamide gel electrophoresis routinely determined no detectable protein other than type I collagen.
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