Example sentences for: dlg
How can you use “dlg” in a sentence? Here are some example sentences to help you improve your vocabulary:
The exons and flanking intronic sequences of DLG50 were amplified by PCR and sequenced in four members of the family, two affected and two normals.
There was considerable overlap between the 305 bp fragment of the new sequence and that of the published cDNA of DLG5.
The DLG5 gene encodes for a protein that is a member of the MAGUK (Membrane Associated Guan late Kinase homologs) family of proteins located in the plasma membrane [ 2 ] . MAGUK is a new family of proteins that act as a molecular scaffold for intracellular signaling pathways [ 11 ] . The MAGUK family of proteins is characterized by domains that interact with other proteins to create an assembly of large multi-protein complexes [ 10 ] . The PDZ (PSD-95, DLG and ZO-1) domains are the best characterized for their binding to the C-terminal region of channels and transmembrane proteins [ 11 ] . Usually each protein has 1-3 copies of such domains [ 8 12 13 ] . The SH3 domain (Src homology 3) are present in proteins that couple transmembrane receptors to signaling molecules [ 14 ] . The presence of this domain in MAGUKs increases their possibilities of interacting with signaling pathways.
The MAGUK family of proteins [ 2 10 ] have characteristic domains which are present in DLG5: PDZ1 at bp 3763-4074, PDZ 2 domain 4186-4518 bp, SH3 domain bp 4570-4782 and the GUK domain at bp 4915-5539 (Figure 2).
We then performed PCR using primers anchored in the introns of DLG5 for each of the exons on both the normal haploid DNA and the disease bearing haploid DNA.
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