Example sentences for: discontinuous

How can you use “discontinuous” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The adenoviral vector DNA were linerized with Pac I and transfected into the replication-permissive 293 cells (E1A transcomplementing cell line) using Lipofectamine (Life Technologies) to produce E1-deleted, replication-defective recombinant adenovirus as described previously [ 30 ] . Large-scale amplification of the recombinant adenovirus in 293 cells was followed by purification using a discontinuous CsCl gradient.

  • A discontinuous layer of an electron dense substance was observed beneath the boarder between the endothelial layer and Descemet's membrane (fig 5).

  • Samples of known numbers of cells from the cell lines and recombinant, purified Tag prepared from a baculovirus lysate [ 40 ] were loaded on 10% polyacrylamide discontinuous mini-gels (Bio-Rad, Hercules, CA) and electrophoresed conventionally [ 41 ] . Gels were then electrophoretically blotted [ 42 ] onto PVDF membrane (Bio-Rad) without methanol for 15 min at 100 V. Tag was visualized by immunostaining with PAb416 and alkaline phosphatase conjugated secondary antibody (Sigma) using chemiluminescent detection with CDP-Star substrate according to the manufacturers directions (Tropix, Bedford, MA).

  • We report here on the structural, functional, and evolutionary relationships of moeZ and discuss the possibility that the discontinuous distribution of moeZ is the result of a LGT event between unrelated bacteria.

  • Moreover, the electron micrographs show that nuclei containing clusters of inclusions often have regions where the nuclear membrane appears to be discontinuous (Fig.


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