Words similar to discontinuous
Example sentences for: discontinuous
How can you use “discontinuous” in a sentence? Here are some example sentences to help you improve your vocabulary:
Synchronous discontinuous operations were performed in all cases.
The lysates were centrifuged at 4000 × g for 15 minutes, then aliquoted and stored at -20°C prior to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and western analysis as previously described [ 12 ] . Protein concentrations of the cell lysates were determined using the Bio-Rad Protein Assay (Hercules, CA), and sample volumes adjusted so that 5-10 μg of total protein were loaded into wells of a discontinuous (12% separating, 4% stacking) polyacrylamide gel.
The adenoviral vector DNA were linerized with Pac I and transfected into the replication-permissive 293 cells (E1A transcomplementing cell line) using Lipofectamine (Life Technologies) to produce E1-deleted, replication-defective recombinant adenovirus as described previously [ 30 ] . Large-scale amplification of the recombinant adenovirus in 293 cells was followed by purification using a discontinuous CsCl gradient.
A 12% discontinuous SDS/polyacrylamide gel was used for molecular weight and purity analysis [ 17 ] .
A discontinuous layer of an electron dense substance was observed beneath the boarder between the endothelial layer and Descemet's membrane (fig 5).