Words similar to discontinuous
Example sentences for: discontinuous
How can you use “discontinuous” in a sentence? Here are some example sentences to help you improve your vocabulary:
The adenoviral vector DNA were linerized with Pac I and transfected into the replication-permissive 293 cells (E1A transcomplementing cell line) using Lipofectamine (Life Technologies) to produce E1-deleted, replication-defective recombinant adenovirus as described previously [ 30 ] . Large-scale amplification of the recombinant adenovirus in 293 cells was followed by purification using a discontinuous CsCl gradient.
A discontinuous layer of an electron dense substance was observed beneath the boarder between the endothelial layer and Descemet's membrane (fig 5).
Samples of known numbers of cells from the cell lines and recombinant, purified Tag prepared from a baculovirus lysate [ 40 ] were loaded on 10% polyacrylamide discontinuous mini-gels (Bio-Rad, Hercules, CA) and electrophoresed conventionally [ 41 ] . Gels were then electrophoretically blotted [ 42 ] onto PVDF membrane (Bio-Rad) without methanol for 15 min at 100 V. Tag was visualized by immunostaining with PAb416 and alkaline phosphatase conjugated secondary antibody (Sigma) using chemiluminescent detection with CDP-Star substrate according to the manufacturers directions (Tropix, Bedford, MA).
We report here on the structural, functional, and evolutionary relationships of moeZ and discuss the possibility that the discontinuous distribution of moeZ is the result of a LGT event between unrelated bacteria.
Moreover, the electron micrographs show that nuclei containing clusters of inclusions often have regions where the nuclear membrane appears to be discontinuous (Fig.
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