Words similar to dii
Example sentences for: dii
How can you use “dii” in a sentence? Here are some example sentences to help you improve your vocabulary:
Ears were subsequently reacted for acetylated tubulin to reveal the pattern of innervation in addition to the DiI tracing with a different technique as previously described [ 14 ] . Briefly, dissected ears were incubated with 1:500 anti-acetylated tubulin antibodies (Sigma, St. Louis) followed by secondary antibodies conjugated to HRP.
High levels of BMP-4 mRNA continue to be expressed in the TE of the CL throughout luteogenes, i.e. luteinization (E 0200 h to DI 1100 h), and luteolysis (DII 1100 h to E 0200 h the next cycle) (Fig.
DiI tracing from the brainstem was performed as previously described [ 41 ] . Small filter stripes soaked with saturated DiI were implanted into the efferent fiber bundle near the floor plate or into the ascending or descending afferents in the alar plate [ 42 ] . After appropriate diffusion time of the dye, the ears were dissected and mounted flat for visualization in an epifluorescent microscope.
To visualize lipid membranes, sections were stained with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate ('DiI';DiIC 18 ; Molecular Probes) as previously described [ 24 ] . Sections were then washed several times with Tris buffered saline, and nucleic acids were stained by incubating sections in TBS containing 1 μM SYTOX Green (Molecular Probes) for 10 min at RT followed by several washes with TBS prior to confocal imaging.
Examination of mid-axial sections stained with DiI and SYTOX green revealed unique differences in gross morphology and nucleic acid staining between alphaA/BKO lenses and wild type lenses (Figure 2).
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