Words similar to difluoride
Example sentences for: difluoride
How can you use “difluoride” in a sentence? Here are some example sentences to help you improve your vocabulary:
PIP3: Phosphatidyl-3,4,5-triphosphate; DMEM: Dulbecco's Modified Eagle's Medium; PI-3Kinase: phosphatidylinositol-3-kinase; MAPK: mitogen-activated protein kinase; IGF: insulin-like growth factor; PTEN: phosphatase and tensin homolog deleted from chromosome 10; SOS: son-of-sevenless ras exchange factor; ASK: apoptosis stimulating kinase; FKHR: forkhead related protein; cFLIP: cellular FLICE interacting protein; BSA: bovine serum albumin; TBS: tris buffered saline; PVDF: polyvinylidene difluoride
For in vitro protein-protein interaction assays, 5 μg of either GST-CAF1 or GST purified proteins (prepared as described in [ 25 ] ) were subjected to 10% SDS/PAGE and transferred onto a polyvinylidene difluoride membrane (Millipore) by electroblotting.
The extracts, normalized for total protein content, were separated by SDS/PAGE and transferred to a polyvinylidene difluoride membrane (Immobilon-P, Millipore Corp.).
Total cellular protein was separated on 4-20% Tris-glycine gels (Novex, Inc.) and transferred to a polvinylidene difluoride (PVDF) membranes (Immobilon-P transfer membranes; Millipore Corp.) overnight at 0.08 A. Following the transfer, blots were blocked with 5% non-fat dry milk in 50 ml of TNE 50 (100 mM Tris-Cl [pH 8.0], 50 mM NaCl, 1 mM EDTA) plus 0.1% NP-40.
DMSO (dimethyl sulfoxide); EGTA (ethylene glycol bis-α minoethylether tetraacetic acid); HEPES (2-hydroxylethyl piperazine 2-ethane sulfonic acid); MOPS (3N-Morpholino-propane sulfonic acid); PVDF (polyvinylidene difluoride); TTX (tetrodotoxin); TEA (tetraethylamonium); ORF (origin of replication).
Loading...