Words similar to difluoride
Example sentences for: difluoride
How can you use “difluoride” in a sentence? Here are some example sentences to help you improve your vocabulary:
BCIP = 5-bromo-4-chloroindol-3-yl phosphate; cDNA = complementary DNA; EGTA = (ethylene-bis [oxyethylenenitrilo])tetraacetic acid; CHTN = Cooperative Human Tissue Network; ELISA = enzyme-linked immunosorbent assay; MAP = microtubule associated protein; MES = 2-( N -morpholino)-ethane sulfonic acid; NBT = nitro blue tetrazolium; PBS = phosphate-buffered saline; PC-tubulin = MAP-free phosphocellulose-purified tubulin; PCR = polymerase chain reaction; PIPES = piperazine- N,N '-bis(2-ethanesulfonic acid); PVDF = poly(vinylidene difluoride).
The gel was then equilibrated in transfer buffer for 15 min and transferred to a polyvinylidine difluoride membrane (Novex, San Diego, CA), also equilibrated in transfer buffer for 15 min, for 1 hr at 100 V. The membrane was blocked overnight with 5% non-fat milk in PBS at 4°C, and incubated with a rabbit polyclonal antibody (1:2000) against a peptide (CPRPNREEPVDSRTP) at the C-terminal region of MECP2 (Upstate Biotechnology, Lake Placid, NY) at room temperature for 2 h in a western wash solution (PBS containing 2% non-fat milk, 0.1% Tween 20, and 350 mM NaCl).
The extracts, normalized for total protein content, were separated by SDS/PAGE and transferred to a polyvinylidene difluoride membrane (Immobilon-P, Millipore Corp.).
Total cellular protein was separated on 4-20% Tris-glycine gels (Novex, Inc.) and transferred to a polvinylidene difluoride (PVDF) membranes (Immobilon-P transfer membranes; Millipore Corp.) overnight at 0.08 A. Following the transfer, blots were blocked with 5% non-fat dry milk in 50 ml of TNE 50 (100 mM Tris-Cl [pH 8.0], 50 mM NaCl, 1 mM EDTA) plus 0.1% NP-40.
Samples were boiled in 1× Laemmli buffer for 3 minutes, separated by SDS-PAGE, and blotted to polyvinylidene difluoride membranes.
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