Words similar to difluoride
Example sentences for: difluoride
How can you use “difluoride” in a sentence? Here are some example sentences to help you improve your vocabulary:
AID (α subunit interaction domain); BID (β subunit interaction domain); DMSO (dimethyl sulfoxide); EGTA (ethylene glycol bis-aminoethylether tetraacetic acid); HEPES (2-hydroxylethyl piperazine 2-ethane sulfonic acid); MOPS (3N-Morpholino-propane sulfonic acid); PVDF (polyvinylidene difluoride); TTX (tetrodotoxin); TEA (tetraethylamonium).
These samples were separated by SDS-PAGE, electrophoretically transferred to polyvinylidene difluoride membrane, and blocked with 5% milk in PBS-T.
PIP3: Phosphatidyl-3,4,5-triphosphate; DMEM: Dulbecco's Modified Eagle's Medium; PI-3Kinase: phosphatidylinositol-3-kinase; MAPK: mitogen-activated protein kinase; IGF: insulin-like growth factor; PTEN: phosphatase and tensin homolog deleted from chromosome 10; SOS: son-of-sevenless ras exchange factor; ASK: apoptosis stimulating kinase; FKHR: forkhead related protein; cFLIP: cellular FLICE interacting protein; BSA: bovine serum albumin; TBS: tris buffered saline; PVDF: polyvinylidene difluoride
Total cellular protein was separated on 4-20% Tris-glycine gels (Novex, Inc.) and transferred to a polvinylidene difluoride (PVDF) membranes (Immobilon-P transfer membranes; Millipore Corp.) overnight at 0.08 A. Following the transfer, blots were blocked with 5% non-fat dry milk in 50 ml of TNE 50 (100 mM Tris-Cl [pH 8.0], 50 mM NaCl, 1 mM EDTA) plus 0.1% NP-40.
Proteins were separated by SDS-PAGE and transferred to a polyvinylidene difluoride membrane (Roche) as described previously [ 66 ] . Blot membranes were blocked for 3 h at 37°C in PBS containing 5 % skim milk and probed with the respective antibodies (16 h at 4°C).
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