Words similar to difluoride
Example sentences for: difluoride
How can you use “difluoride” in a sentence? Here are some example sentences to help you improve your vocabulary:
The proteins separated by SDS-PAGE were transferred electrophoretically to poly(vinylidene difluoride) membranes (Bio-Rad).
Samples were boiled in 1× Laemmli buffer for 3 minutes, separated by SDS-PAGE, and blotted to polyvinylidene difluoride membranes.
The gel was then equilibrated in transfer buffer for 15 min and transferred to a polyvinylidine difluoride membrane (Novex, San Diego, CA), also equilibrated in transfer buffer for 15 min, for 1 hr at 100 V. The membrane was blocked overnight with 5% non-fat milk in PBS at 4°C, and incubated with a rabbit polyclonal antibody (1:2000) against a peptide (CPRPNREEPVDSRTP) at the C-terminal region of MECP2 (Upstate Biotechnology, Lake Placid, NY) at room temperature for 2 h in a western wash solution (PBS containing 2% non-fat milk, 0.1% Tween 20, and 350 mM NaCl).
For in vitro protein-protein interaction assays, 5 μg of either GST-CAF1 or GST purified proteins (prepared as described in [ 25 ] ) were subjected to 10% SDS/PAGE and transferred onto a polyvinylidene difluoride membrane (Millipore) by electroblotting.
PIP3: Phosphatidyl-3,4,5-triphosphate; DMEM: Dulbecco's Modified Eagle's Medium; PI-3Kinase: phosphatidylinositol-3-kinase; MAPK: mitogen-activated protein kinase; IGF: insulin-like growth factor; PTEN: phosphatase and tensin homolog deleted from chromosome 10; SOS: son-of-sevenless ras exchange factor; ASK: apoptosis stimulating kinase; FKHR: forkhead related protein; cFLIP: cellular FLICE interacting protein; BSA: bovine serum albumin; TBS: tris buffered saline; PVDF: polyvinylidene difluoride
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