Example sentences for: difluoride

How can you use “difluoride” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Total cellular protein was separated on 4-20% Tris-glycine gels (Novex, Inc.) and transferred to a polvinylidene difluoride (PVDF) membranes (Immobilon-P transfer membranes; Millipore Corp.) overnight at 0.08 A. Following the transfer, blots were blocked with 5% non-fat dry milk in 50 ml of TNE 50 (100 mM Tris-Cl [pH 8.0], 50 mM NaCl, 1 mM EDTA) plus 0.1% NP-40.

  • The proteins separated by SDS-PAGE were transferred electrophoretically to poly(vinylidene difluoride) membranes (Bio-Rad).

  • AID (α subunit interaction domain); BID (β subunit interaction domain); DMSO (dimethyl sulfoxide); EGTA (ethylene glycol bis-aminoethylether tetraacetic acid); HEPES (2-hydroxylethyl piperazine 2-ethane sulfonic acid); MOPS (3N-Morpholino-propane sulfonic acid); PVDF (polyvinylidene difluoride); TTX (tetrodotoxin); TEA (tetraethylamonium).

  • For in vitro protein-protein interaction assays, 5 μg of either GST-CAF1 or GST purified proteins (prepared as described in [ 25 ] ) were subjected to 10% SDS/PAGE and transferred onto a polyvinylidene difluoride membrane (Millipore) by electroblotting.

  • Gels were transferred in 20% methanol to polyvinylidene difluoride membrane (Amersham Pharmacia Biotech, Piscataway, NJ, USA).


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