Words similar to dialyzed

Example sentences for: dialyzed

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Vectors were purified using two consecutive CsCl gradient ultracentrifugation, dialyzed at 4°C against sterile virus buffer, aliquoted, and stored at -80°C until use.
The resulting protein pellet was resuspended in 10 ml of buffer A (50 mM Tris-HCl, pH 7.5, 5 mM MgCl 2, 0.1 mM EDTA, 0.5 mM DTT, 10 % glycerol and 1 mM PMSF) and dialyzed against 500 ml of buffer A overnight.
The fractions containing approximately equal band intensity of p66 and p51 were dialyzed against a storage buffer (50 mM Tris HCl pH 7.0, 200 mM NaCl and 50% Glycerol) and this enzyme preparation was used in all experiments.
To identify components with PEDF-binding affinity, purified recombinant protein was used to prepare PEDF-affinity resin with 3 M Emphaze™ Biosupport Medium (Pierce Chemical) [ 18 ] . CM awas dialyzed against buffer P (20 mM sodium phosphate pH 7, 150 mM NaCl, 0.5% CHAPS) and filtrated through 0.4 μm filters.
The concentrated preparation was dialyzed overnight against 50 mM phosphate buffer (pH 7.0) containing 1 mM DTT.