Example sentences for: dialyzed

How can you use “dialyzed” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Eluted protein was concentrated by dialysis against powdered polyethylene glycol (Aquacide; Calbiochem), then dialyzed into 20% glycerol, 20 mM Tris pH 7.5, 20 mM KCl, 1 mM EDTA, 1 mM DTT.

  • Vectors were purified using two consecutive CsCl gradient ultracentrifugation, dialyzed at 4°C against sterile virus buffer, aliquoted, and stored at -80°C until use.

  • To identify components with PEDF-binding affinity, purified recombinant protein was used to prepare PEDF-affinity resin with 3 M Emphaze™ Biosupport Medium (Pierce Chemical) [ 18 ] . CM awas dialyzed against buffer P (20 mM sodium phosphate pH 7, 150 mM NaCl, 0.5% CHAPS) and filtrated through 0.4 μm filters.

  • The mixture was packed into a 10 ml Polyprep chromatography column (Bio-Rad) and washed with 10-column volumes of buffer P. The bound material was eluted with 10-column volumes of 3 M NaCl, desalted with 20 mM Tris-HCl pH 8.0, 10% glycerol, treated for protein depletion, dialyzed against deionized water, lyophilized and resuspended in deionized water.

  • The concentrations of L1 and the mutants were determined by measuring the proteins' absorbance at 280 nm and using the published extinction coefficient of ε 280 nm = 54,804 M -1•cm -1 [ 36 ] or by using the method of Pace [ 69 ] . Before metal analyses, the protein samples were dialyzed versus 3 × 1 L of metal-free, 50 mM HEPES, pH 7.5 over 96 hours at 4°C.


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