Example sentences for: dfz
How can you use “dfz” in a sentence? Here are some example sentences to help you improve your vocabulary:
In this experiment, a Wg plasmid activated the luciferase reporter only 12-fold above the vector control; wild-type DFz2 plasmid alone stimulated luciferase levels 7-fold, but neither deletion mutant of DFz2 had any effect, consistent with an earlier report that DFz2 with a truncated carboxyterminus fails to induce the expression of Xnr/Siamois [ 21 ] . However, DFz2ΔC stimulated signaling over 700-fold when delivered in concert with Wg, a synergistic effect nearly as strong as observed with wild type DFz2 (nearly 1700-fold in this experiment).
In earlier reports, Northern blot hybridization and RT-PCR failed to detect DFz1, DFz2, and DFz3 in S2 cells [ 6 17 18 ] . Using a one-step RT-PCR assay to measure the expression levels of all 4 DFzs and Arr in the same S2 cells used by Yanagawa et al . (Figure 1A), we detected DFz2, DFz3, and Arr mRNAs, but not DFz1 and DFz4 mRNAs after 40 cycles of amplification.
Two others (DFz2 and 3) are detectable (Figure 1A), but DFz2 is in limiting quantities, since signaling by Wg, the Drosophila ortholog of mammalian Wnt-1, can be dramatically stimulated by co-transfection with a DFz2 expression plasmid (Figure 1B).
Signaling via LRP6ΔN, however, while dependent on Arm as expected, is not dependent on Dsh or DFz2, as judged by the use of dsRNAi (Figure 5A).
Importantly, the synergistic effect of co-transfected Wg and DFz2 plasmids, approximately 1000-fold, was also dramatically reduced by addition of inhibitory RNA against Arr (Figure 2B), implying that endogenous Arr is required for the stimulatory effect of exogenous DFz2 on Wg signaling.
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