Words similar to destaining

Example sentences for: destaining

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The gels were then incubated for 24 hours at 37°C in 50 mM Tris-HCl buffer, pH 7.4, containing 5 mM CaCl 2 . A duplicate negative control gel was incubated as described above but in 50 mM Tris-HCl, pH 7.4 containing 10 mM EDTA instead of CaCl 2 . The gels were fixed for 1 hour in 40% methanol /7% acetic acid and then stained in Coomassie Blue Solution (Sigma), followed by destaining in 10% methanol, 7% acetic acid.
The membrane was removed, washed twice with destaining solution (ethanol-water-acetic acid, 50:49:1) for 5 min and air-dried.
Proteins were electroeluted from the non-denaturing gel as describe here [ 24 ] . Gels were fixed with fixing solution (50% methanol, 10% acetic acid) for 15 minutes, followed by staining in Coomassie staining solution (20% acetic acid, 0.025% Coomassie blue G-250) for one hour, followed by destaining in destaining solution (5% methanol, 10% acetic acid) [ 42 ] .