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Example sentences for: described

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E15 SMG (mostly Canalicular Stage) primordia were cultured using a modified Trowell method as previously described [ 13 ] . The medium consisted of BGJb (Life Technologies, Rockville, MD) supplemented with 0.5 mg ascorbic acid/ml and 50 units penicillin/streptomycin (Life Technologies), pH 7.2, and replicate cultures were changed every other day.
The La Poste simulation model and the cost function developed for the USPS, described above, provide alternative means of estimating average unit costs over a range of volumes per delivery point and postal densities.
Biotinylated eggs were extracted in Celis buffer and run on a 2D gel as described above.
C3 exoenzyme was purified as previously described [ 25 ] . Anti-β1 integrin mAb 33B6 and anti α4β1 integrin mAb 19H8 were generated in this laboratory and have been previously described [ 27 28 ] . Rabbit polyclonal anti-Cdc42, -Rac1, -Rac2, -RhoA, and mouse monoclonal anti-Ras, were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).
After incubation for 1 h at 37°C, the wells were washed as described above and HRP-streptavidin (125 ng/ml in PBS containing 10% goat serum; 100 μl/well) was added.