Words similar to dek

Example sentences for: dek

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Three specific findings in this report support a potential role for DEK as a transcriptional modulator of MHC class II expression.
Participation of both DEK and NF-Y in the DQA1 Y-box binding complex was confirmed by using high-titer anti-DEK antiserum (gift of W Szer [ 9 ] ) and monoclonal antibody to the NF-YA subunit of the Y-box binding factor (gift of JP-Y Ting) to further retard the mobility of the DQA1 Y-box binding complex ('supershifting') (Fig.
For antibody supershift of binding complexes, 1 μl anti-NF-YA antibody (gift of JP-Y Ting) or 1 μl high-titer anti-DEK human serum (gift of W Szer [ 9 ] ) or 2-3 μl control human serum was added to the binding reaction, and the mixture was incubated on ice for 2 hours before the probe was added.
Consistent protection is also seen at position 2, adjacent to the A-for-G substitution that diverges from the Y-box consensus sequence, although there is no protection of the divergent base itself at position 3. DNase I protection by recombinant DEK extends over the length of the Y-box sequence, including bases within the NF-Y binding site (reverse CCAAT sequence), again suggesting that the two proteins may interact.
The C-terminal region of DEK, which contains the putative DNA-binding domain, appears to be most antigenic [ 34 ] (and K Sitwala and DM Markovitz, unpublished observations), raising the possibility that altered DNA binding may expose other masked epitopes.