Words similar to dek

Example sentences for: dek

How can you use “dek” in a sentence? Here are some example sentences to help you improve your vocabulary:

Three specific findings in this report support a potential role for DEK as a transcriptional modulator of MHC class II expression.
Consistent protection is also seen at position 2, adjacent to the A-for-G substitution that diverges from the Y-box consensus sequence, although there is no protection of the divergent base itself at position 3. DNase I protection by recombinant DEK extends over the length of the Y-box sequence, including bases within the NF-Y binding site (reverse CCAAT sequence), again suggesting that the two proteins may interact.
Recombinant protein was purified from bacterial lysates in accordance with the published method for the QIA express system (Qiagen, Valencia, CA, USA) with variations in Buffers B and D as noted in Additional file: 1. Procedures were carried out at 4°C; dialyzed recombinant DEK protein (rDEK) was stored at -80°C.
For antibody supershift of binding complexes, 1 μl anti-NF-YA antibody (gift of JP-Y Ting) or 1 μl high-titer anti-DEK human serum (gift of W Szer [ 9 ] ) or 2-3 μl control human serum was added to the binding reaction, and the mixture was incubated on ice for 2 hours before the probe was added.
The same electrophoretic patterns were seen after preincubation of nuclear extracts from the CEM T lymphocytoid cell line with anti-DEK and anti-NF-YA antibodies (data not shown).