Words similar to dek
Example sentences for: dek
How can you use “dek” in a sentence? Here are some example sentences to help you improve your vocabulary:
Consistent protection is also seen at position 2, adjacent to the A-for-G substitution that diverges from the Y-box consensus sequence, although there is no protection of the divergent base itself at position 3. DNase I protection by recombinant DEK extends over the length of the Y-box sequence, including bases within the NF-Y binding site (reverse CCAAT sequence), again suggesting that the two proteins may interact.
Finally, DEK binds differentially to specific Y-box sequences found in HLA-DQA1(*0101 and *0501 alleles) and HLA-DRA , consistent with observations of DQA1 gene-specific cell-surface expression [ 36 ] and allele-specific promoter activity [ 33 ] . The specificity of rDEK binding to these and other, related Y-box sequences (including HLA-DQB and HLA-DRB alleles associated with the DR4 haplotype) may correlate with a predisposition to autoim-mune disease seen with certain HLA haplotypes.
Recombinant protein was purified from bacterial lysates in accordance with the published method for the QIA express system (Qiagen, Valencia, CA, USA) with variations in Buffers B and D as noted in Additional file: 1. Procedures were carried out at 4°C; dialyzed recombinant DEK protein (rDEK) was stored at -80°C.
The C-terminal region of DEK, which contains the putative DNA-binding domain, appears to be most antigenic [ 34 ] (and K Sitwala and DM Markovitz, unpublished observations), raising the possibility that altered DNA binding may expose other masked epitopes.
The same electrophoretic patterns were seen after preincubation of nuclear extracts from the CEM T lymphocytoid cell line with anti-DEK and anti-NF-YA antibodies (data not shown).
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