Example sentences for: dek

How can you use “dek” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • DNase I footprinting with recombinant protein further defined the physical interaction between DEK and the HLA-DQA1*0101 Y-box element (Fig.

  • For antibody supershift of binding complexes, 1 μl anti-NF-YA antibody (gift of JP-Y Ting) or 1 μl high-titer anti-DEK human serum (gift of W Szer [ 9 ] ) or 2-3 μl control human serum was added to the binding reaction, and the mixture was incubated on ice for 2 hours before the probe was added.

  • We hypothesized that DEK could bind in a sequence-specific manner to the Y-box motifs in the promoter regions of several class II MHC genes, and that gene- and allele-specific Y-box polymorphisms could affect DEK binding activity.

  • A FLAG-tagged DEK adenoviral vector constructed by the University of Michigan Vector Core was used to transduce T98G cells (ATCC) by incubation for 48 hours before harvesting for immunoprecipitation.

  • Transient transfection experiments in cultured cell lines have not proved useful in examining this model, for DEK is highly expressed in most cells of hematopoietic lineage, and further overexpression has resulted in apparently nonspecific downregulation of transcriptional activity (as might be predicted from [ 14 ] ). For this reason, we are currently pursuing other experimental approaches.

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