Words similar to deab
Example sentences for: deab
How can you use “deab” in a sentence? Here are some example sentences to help you improve your vocabulary:
Treatment of L1210/CPA cells in vitro with 50 μM DEAB abolished the tumor cells' resistance to 4-HC [ 19 ] . DEAB also exhibited in vivo efficacy as a ALDH1A1 inhibitor, as demonstrated by the toxicity to the intestinal crypt cells in mice receiving co-injections of DEAB and cyclophosphamide [ 21 ] . More recently, DEAB has been used to demonstrate that overexpression of human ALDH1A1 in transfected cell lines is sufficient to cause cellular resistance to the oxazaphosphorines [ 22 ] and 4-HC [ 23 ] . DEAB has also been an effective reagent in fluorescent-activated cell sorting (FACS) techniques to isolate human hematopoietic stem cells based on the expression of ALDH1A1 activity [ 5 6 ] . In contrast to DEAB and DPAB, daidzin - a natural product isolated from Pueraria lobata (the Kudzu plant) - shows specificity for inhibiting ALDH2 at concentrations similar to that of DPAB used to inhibit ALDH1A1 [ 24 25 ] . An inhibitor specific for ALDH1A2 has not yet been reported.
By day 6, less than 1% of the retinal was oxidized to retinoic acid, suggesting that non-enzymatic formation or serum-mediated formation of retinoic acid was not responsible for the observed differentiation in cells exposed to DEAB or DPAB.
With co-exposures to 1 μM retinal and up to 50 μM DEAB or DPAB, HL-60 differentiation was inhibited by only 30% after 144 hours of exposure, compared with cells exposed to retinal alone (Figure 2) DPAB did not alter the induction of differentiation by retinoic acid, nor did it affect cell viability up to 144 hours.
In addition, the NAD-dependent oxidation of propanal or retinal was less than 0.05 nmoles NADH formed/min-10 7cells in spectrophotometric assays using HL-60 cell extracts, even in the absence of DEAB or DPAB.
Although DPAB is a more potent inhibitor of human ALDH1A1 than DEAB, we were concerned that DPAB was not remaining in solution at 50 μM in the culture media.
Loading...