Words similar to dctp
Example sentences for: dctp
How can you use “dctp” in a sentence? Here are some example sentences to help you improve your vocabulary:
A 340-bp fragment of an LCP1 cDNA was radiolabeled with [α 32P]-dCTP using a random primer labeling kit (Roche, Indianapolis, IN).
The radioactively labeled PCR products were separated on 6% denaturing polyacrylamide sequencing gels, and the alleles were visualized by autoradiography [ 6 ] . For the fluorescently labeled products, α- 32P-dCTP was omitted, fluorescent primers were used, and the products were analyzed in an ABI PrismTM 377 DNA Sequencer (Perkin Elmer) following the manufacturer's specifications.
5-methyl dCTP was used during first-strand cDNA synthesis to protect internal Xho I sites.
Clones were selected at random and used as α 32P-dCTP (Amersham Biosciences Corp, Piscataway, NY, USA) probes on dot blots or Southern blots containing amplicon DNA from the original normal and metastatic cells to verify whether these sequences are actually present in the normal cell DNA sample and missing in the metastatic cell DNA samples.
[ 53], including 32P-dCTP (40 mCi/ml; NEN Life Science Products, Boston, MA) in the reaction mixture.
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