Example sentences for: dctp

How can you use “dctp” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 6 Ci/mmol), [ 32P]-dCTP (3000 Ci/mmol), and [ 32P]-ATP (3000 Ci/mmol) were purchased from Amersham Pharmacia Biotech (Baie d'Urfe, QC, Canada).

  • 1 M), d(GAT)TP (25 mM), dCTP (1 mM), Cy3-dCTP (2 mM)(Amersham, Piscataway, NJ) and Superscript RT II (200 units) were added and incubated at room temperature for 10 minutes and then at 42°C for 2 hours.

  • In the first DNA-protein binding reaction, [ 32P]-labeled double stranded oligonucleotides were generated by first annealing BSS2 (50 ng) to the BSS4 oligonucleotide (500 ng) then end-filling with 250 μM each of dATP, dGTP, and dTTP, 50 μCi of 32P-dCTP and Klenow.

  • Reverse transcription was performed in the presence of 500 μM each of dATP, dCTP, and dGTP, 200 μM 5-aminoallyl-dUTP (Sigma), 300 μM dTTP, 1 × first-strand buffer, 10 mM dithiothreitol, and 400 U Superscript II (Life Technologies) in 40-μl reactions at 42°C for 3 h to overnight.

  • Reverse transcriptase activity in viral cores was assayed by incubating cores in endogenous polymerase buffer with 40 μM dATP, dCTP, dTTP and 2 μCi [α 32P]dGTP (Amersham) at 37°C for 90 min.


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