Example sentences for: dctp

How can you use “dctp” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The bands were excised from 0.8% low-melt agarose and 20 ng of DNA were labeled with [α- 32P]-dCTP using the Life Technologies Random Primers DNA Labeling System.

  • These 100 clones were probed (labeled with α 32P-dCTP) on dot blots containing normal cell DNA and metastatic cell DNA.

  • The RNA was cross-linked to the membrane by exposure to UV light and hybridized with specific probes labeled with 32P-dCTP (Perkin Elmer Life Sciences, Boston, MA) using the Prime-It random prime labeling kit (Stratagene, La Jolla, CA).

  • To generate labeled single-stranded cDNA target, 10 μg total RNA from rat heart or brain (Clontech, Palo Alto, CA) was reverse transcribed for 2-3 h at 42°C in the presence of 6 μg random primers (Invitrogen/Life Technologies), 1x first-strand synthesis buffer (Invitrogen/Life Technologies), 10 mM DTT, dNTP mix (25 mM dATP, 25 mM dCTP, 25 mM dGTP, 15 mM dTTP, 10 mM amino allyl-dUTP), and 200 units Superscript II reverse transcriptase (Invitrogen/Life Technologies).

  • With direct labeling, Cy3-dCTP is incorporated into control and Cy5-dCTP into tester DNA during the PCR amplification of the hybridized DNA templates.

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