Example sentences for: dctp

How can you use “dctp” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Labeled probe was synthesized using a High Prime random priming kit, according to the manufacturer's instructions (Roche Molecular Biochemicals, Indianapolis, IN), from 100 ng of genomic PCR product and 50 μCi [α- 32p]dCTP.

  • For each gene, stock solutions were prepared containing 1.5 mM MgCl 2 , 1X reaction buffer, 10 μCi of 32P- dCTP (3000 Ci/mmol) (NEN, Boston, MA), 1 μM each primer, and 1.5 U of Taq polymerase (Life Technologies).

  • With direct labeling, Cy3-dCTP is incorporated into control and Cy5-dCTP into tester DNA during the PCR amplification of the hybridized DNA templates.

  • 5-methyl dCTP was used during first-strand cDNA synthesis to protect internal Xho I sites.

  • DNA probes were labeled with [α- 32P]dCTP with the random primer labeling kit (Pharmacia Biotech Inc., NJ).


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