Example sentences for: dctp

How can you use “dctp” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Detailed procedures for isolation of total RNA, enrichment of poly (A)+ RNA by oligo-dT cellulose chromatography and electrophoresis and Northern blotting of RNA have been described previously [ 13 ] . The cDNA probes of mouse RAR α and RAR γ [ 21 22 ] were isolated by agarose electrophoresis after appropriate endonucleases digestion and were radio-labeled with 32P-dCTP, using random priming kit (Boehringer-Mannheim, Indianapolis, IN).

  • A 340-bp fragment of an LCP1 cDNA was radiolabeled with [α 32P]-dCTP using a random primer labeling kit (Roche, Indianapolis, IN).

  • These 100 clones were probed (labeled with α 32P-dCTP) on dot blots containing normal cell DNA and metastatic cell DNA.

  • Small samples (2 μg) from each RNA fraction were reverse transcribed and radiolabeled (2 μg oligo dT, 1x First Strand buffer, 5 mM DTT, 75 U SuperscriptII [Life Technologies], 20 mM dNTP mix containing dATP, dGTP, dTTP and 100 mCi 33P-dCTP (ICN Radiochemicals).

  • [ 3H]Myristic acid and [α- 32P]dCTP were from Dupont-NEN (Boston, MA).


How many words do you know? Try our free vocabulary size test!


Search

Search for example sentences

Loading Loading...
Quantcast