Example sentences for: dctp

How can you use “dctp” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • With direct labeling, Cy3-dCTP is incorporated into control and Cy5-dCTP into tester DNA during the PCR amplification of the hybridized DNA templates.

  • The purified 5 μg aRNA was labeled with Cy3 or Cy5 by reverse transcription in a solution containing 10 μg of random hexamer, 4 μl first-strand reaction buffer, 2 μl 10 mM dithiothreitol (DTT; Gibco-BRL), 1 μl of 2 mM dATP, dGTP, dTTP and 1 mM dCTP, 1 μl SUPERase-in, (Amtion) and 200 u Superscript II reverse transcriptase (Gibco-BRL).

  • [ 3H]Myristic acid and [α- 32P]dCTP were from Dupont-NEN (Boston, MA).

  • PCR labeling was by incorporation of alpha 32P-dCTP, using 10 ng of gel isolated PCR product as a template.

  • Subsequently, a portion of the DNA was labeled with [ 32P]dCTP and used for the next round of site-selection.


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