Words similar to dctp
Example sentences for: dctp
How can you use “dctp” in a sentence? Here are some example sentences to help you improve your vocabulary:
For each gene, stock solutions were prepared containing 1.5 mM MgCl 2 , 1X reaction buffer, 10 μCi of 32P- dCTP (3000 Ci/mmol) (NEN, Boston, MA), 1 μM each primer, and 1.5 U of Taq polymerase (Life Technologies).
Clones were selected at random and used as α 32P-dCTP (Amersham Biosciences Corp, Piscataway, NY, USA) probes on dot blots or Southern blots containing amplicon DNA from the original normal and metastatic cells to verify whether these sequences are actually present in the normal cell DNA sample and missing in the metastatic cell DNA samples.
2 , 0.4 μM TNF-α primer or COX-2 primer, 1:19 ratio of 18S competimers/primers, 0.1 mM each dNTP, 0.2 μl α [ 32P] dCTP (10 mCi/ml; > 3000 Ci/mmol) and 0.025 units of Amplitaq (Perkin Elmer Inc., Norwalk, CT, USA).
The RNA was cross-linked to the membrane by exposure to UV light and hybridized with specific probes labeled with 32P-dCTP (Perkin Elmer Life Sciences, Boston, MA) using the Prime-It random prime labeling kit (Stratagene, La Jolla, CA).
The radiolabeled nucleotide [α- 32P]dCTP (3,000 Ci/mmol) and nick translation kits were purchased from Amersham-Pharmacia.
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