Example sentences for: dctp

How can you use “dctp” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Labeled probe was synthesized using a High Prime random priming kit, according to the manufacturer's instructions (Roche Molecular Biochemicals, Indianapolis, IN), from 100 ng of genomic PCR product and 50 μCi [α- 32p]dCTP.

  • 1 M), d(GAT)TP (25 mM), dCTP (1 mM), Cy3-dCTP (2 mM)(Amersham, Piscataway, NJ) and Superscript RT II (200 units) were added and incubated at room temperature for 10 minutes and then at 42°C for 2 hours.

  • For each gene, stock solutions were prepared containing 1.5 mM MgCl 2 , 1X reaction buffer, 10 μCi of 32P- dCTP (3000 Ci/mmol) (NEN, Boston, MA), 1 μM each primer, and 1.5 U of Taq polymerase (Life Technologies).

  • The bands were excised from 0.8% low-melt agarose and 20 ng of DNA were labeled with [α- 32P]-dCTP using the Life Technologies Random Primers DNA Labeling System.

  • 5-Amino-propargyl-2'-deoxycytidine 5'-triphosphate coupled to Cy3 (Cy5) fluorescent dye - Cy 3-AP3-dCTP or Cy5 -AP3-dCTP (Cy3 and Cy5) were purchased from Amersham Pharmacia Biotech Inc. (Piscataway, NJ).


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