Words similar to dctp
Example sentences for: dctp
How can you use “dctp” in a sentence? Here are some example sentences to help you improve your vocabulary:
For each sample, 50 μL solution containing approximately 5 units of Klenow large fragment (New England Biolabs, Beverly, MA), 3 μg of single stranded binding protein (US Biochemicals, Cleveland, OH), and 0.5 μM Cy3- or Cy5-labeled dATP, dCTP, dGTP or dUTP (Perkin Elmer) was pipeted onto the gel.
With direct labeling, Cy3-dCTP is incorporated into control and Cy5-dCTP into tester DNA during the PCR amplification of the hybridized DNA templates.
The bands were excised from 0.8% low-melt agarose and 20 ng of DNA were labeled with [α- 32P]-dCTP using the Life Technologies Random Primers DNA Labeling System.
[ 3H]Myristic acid and [α- 32P]dCTP were from Dupont-NEN (Boston, MA).
5-Amino-propargyl-2'-deoxycytidine 5'-triphosphate coupled to Cy3 (Cy5) fluorescent dye - Cy 3-AP3-dCTP or Cy5 -AP3-dCTP (Cy3 and Cy5) were purchased from Amersham Pharmacia Biotech Inc. (Piscataway, NJ).