Example sentences for: dctp

How can you use “dctp” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The radioactively labeled PCR products were separated on 6% denaturing polyacrylamide sequencing gels, and the alleles were visualized by autoradiography [ 6 ] . For the fluorescently labeled products, α- 32P-dCTP was omitted, fluorescent primers were used, and the products were analyzed in an ABI PrismTM 377 DNA Sequencer (Perkin Elmer) following the manufacturer's specifications.

  • These 100 clones were probed (labeled with α 32P-dCTP) on dot blots containing normal cell DNA and metastatic cell DNA.

  • The final reaction included 1X First Strand Buffer (Life Technologies), 5 mM DTT (Life Technologies), 1.5 mM dATP, dGTP and dTTP, 1.5 μl Superscript II Reverse Transcriptase (Life Technologies), and 10 μl of 33P-dCTP (ICN, specific activity 3,000 Ci/mmol).

  • In the first DNA-protein binding reaction, [ 32P]-labeled double stranded oligonucleotides were generated by first annealing BSS2 (50 ng) to the BSS4 oligonucleotide (500 ng) then end-filling with 250 μM each of dATP, dGTP, and dTTP, 50 μCi of 32P-dCTP and Klenow.

  • PCR labeling was by incorporation of alpha 32P-dCTP, using 10 ng of gel isolated PCR product as a template.


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