Words similar to dctp
Example sentences for: dctp
How can you use “dctp” in a sentence? Here are some example sentences to help you improve your vocabulary:
Detailed procedures for isolation of total RNA, enrichment of poly (A)+ RNA by oligo-dT cellulose chromatography and electrophoresis and Northern blotting of RNA have been described previously [ 13 ] . The cDNA probes of mouse RAR α and RAR γ [ 21 22 ] were isolated by agarose electrophoresis after appropriate endonucleases digestion and were radio-labeled with 32P-dCTP, using random priming kit (Boehringer-Mannheim, Indianapolis, IN).
A 340-bp fragment of an LCP1 cDNA was radiolabeled with [α 32P]-dCTP using a random primer labeling kit (Roche, Indianapolis, IN).
These 100 clones were probed (labeled with α 32P-dCTP) on dot blots containing normal cell DNA and metastatic cell DNA.
Small samples (2 μg) from each RNA fraction were reverse transcribed and radiolabeled (2 μg oligo dT, 1x First Strand buffer, 5 mM DTT, 75 U SuperscriptII [Life Technologies], 20 mM dNTP mix containing dATP, dGTP, dTTP and 100 mCi 33P-dCTP (ICN Radiochemicals).
[ 3H]Myristic acid and [α- 32P]dCTP were from Dupont-NEN (Boston, MA).
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