Words similar to dctp
Example sentences for: dctp
How can you use “dctp” in a sentence? Here are some example sentences to help you improve your vocabulary:
With direct labeling, Cy3-dCTP is incorporated into control and Cy5-dCTP into tester DNA during the PCR amplification of the hybridized DNA templates.
The purified 5 μg aRNA was labeled with Cy3 or Cy5 by reverse transcription in a solution containing 10 μg of random hexamer, 4 μl first-strand reaction buffer, 2 μl 10 mM dithiothreitol (DTT; Gibco-BRL), 1 μl of 2 mM dATP, dGTP, dTTP and 1 mM dCTP, 1 μl SUPERase-in, (Amtion) and 200 u Superscript II reverse transcriptase (Gibco-BRL).
[ 3H]Myristic acid and [α- 32P]dCTP were from Dupont-NEN (Boston, MA).
PCR labeling was by incorporation of alpha 32P-dCTP, using 10 ng of gel isolated PCR product as a template.
Subsequently, a portion of the DNA was labeled with [ 32P]dCTP and used for the next round of site-selection.
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