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Example sentences for: dcip
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Concentrations of DCIP (oxidized) were calculated using a molar extinction coefficient of ε = 21.
0 mL of sterile 100 mM phosphate buffer (pH 7.5), 2 mL of 100X Nutrient Broth as a biological reductant (Difco), and 1.0 mL of 1 mM DCIP to a sterile 25 mL serum bottle.
In the Viking's gas exchange experiment, water vapor exposed to the Martian soil released O 2 , but possibly only as a result of the activity of powerful soil oxidants that also might have been responsible for the chemical reactivity seen in other Viking biology experiments [ 20 ] . A molecule such as DCIP or XTT might be destroyed by oxidation in the presence of such soils.
Figure 3shows results of measurement of DCIP reduction in an autoclaved control soil and an extraterrestrial surrogate soil (Idaho red sand).
Reduction of XTT was monitored similarly as described for DCIP, except we measured the increase in absorbance at 465 nm resulting from reduction of the colorless oxidized dye to an orange product [ 16 ] .
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