Words similar to dcip

Example sentences for: dcip

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A select group of signature chemicals were extracted from an organic-poor soil (Figure 1) and identified with instruments that eventually can be miniaturized for use on space exploration missions [ 13 21 22 ] . Actual electron transport by living cells was confirmed by use of two specific dyes (DCIP and XTT) that short-circuit respiratory electron transport chains.
DCIP and XTT reduction assays
DCIP, for example, couples directly with NADH or NADPH to form DCIPH 2 and NAD +or NADP +, making it an ideal electron transport chain indicator [ 15 ] . XTT has been used to measure microbial respiratory activity in complex natural environments [ 16 ] . Respiratory dyes have been used commercially for many years in the Biolog™ system to help identify pure cultures of microorganisms by their patterns of oxidation of multiple organic substrates [ 17 ] . Biolog™ also has been used to characterize oxidation capabilities of soil microbial communities [ 18 ] . Rodriquez, et al.
Since DCIP and XTT have different redox potentials, and XTT in our work appears to generally be more easily reduced by soil microbial communities, this shows a need to use more than one respiratory dye in this type of experiment.
0 mL of sterile 100 mM phosphate buffer (pH 7.5), 2 mL of 100X Nutrient Broth as a biological reductant (Difco), and 1.0 mL of 1 mM DCIP to a sterile 25 mL serum bottle.