Words similar to datp

Example sentences for: datp

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Probes were 3'-end labeled with 35S or 33P-dATP using a terminal transferase reaction and hybridization carried out [ 26 ] . Autoradiograms were generated by dipping slides in NTB2 nuclear track emulsion and storing in the dark at 4°C.
The final reaction included 1X First Strand Buffer (Life Technologies), 5 mM DTT (Life Technologies), 1.5 mM dATP, dGTP and dTTP, 1.5 μl Superscript II Reverse Transcriptase (Life Technologies), and 10 μl of 33P-dCTP (ICN, specific activity 3,000 Ci/mmol).
For polyadenylate tailing at the 3' end of the cDNA, 5 μl tailing buffer (200 mmol/l potassium cacodylate [pH 7.2], 4 mmol/l CoCl 2 , 0.4 mmol/l DTT), 2 mmol/l dATP (Roche, Indianapolis, IN, USA), and 10 U terminal transferase (Roche) were added, and incubated at 37°C for 20 min, followed by heat inactivation at 65°C for 10 min.
Small samples (2 μg) from each RNA fraction were reverse transcribed and radiolabeled (2 μg oligo dT, 1x First Strand buffer, 5 mM DTT, 75 U SuperscriptII [Life Technologies], 20 mM dNTP mix containing dATP, dGTP, dTTP and 100 mCi 33P-dCTP (ICN Radiochemicals).
[α- 32P]dATP and [α- 32P]UTP were purchased from Dupont NEN (Boston, MA).