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Example sentences for: datp

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Small samples (2 μg) from each RNA fraction were reverse transcribed and radiolabeled (2 μg oligo dT, 1x First Strand buffer, 5 mM DTT, 75 U SuperscriptII [Life Technologies], 20 mM dNTP mix containing dATP, dGTP, dTTP and 100 mCi 33P-dCTP (ICN Radiochemicals).
Probes were 3'-end labeled with 35S or 33P-dATP using a terminal transferase reaction and hybridization carried out [ 26 ] . Autoradiograms were generated by dipping slides in NTB2 nuclear track emulsion and storing in the dark at 4°C.
The following reagents were added: 6 μl of 5X first strand buffer, 3 μl 0.1 M DTT, 0.7 μl 50X dNTP (25 mM dATP, dCTP, dGTP and 10 mM dTTP), 3 μl 1 mM Cy3-dUTP or Cy5-dUTP and 1.7 μl Superscript™ II (200 U/μl).
Radioactive products were made by 30 cycles of PCR amplification of 10-μl reaction volumes containing 10-30 ng of genomic DNA, 40 ng of each primer, 200 μM each of dATP, dTTP, dGTP, and dCTP, 1 μCi α- 32P-dCTP (3,000 Ci mmol -1) and 0.5 U Taq polymerase (Perkin Elmer).
In the first DNA-protein binding reaction, [ 32P]-labeled double stranded oligonucleotides were generated by first annealing BSS2 (50 ng) to the BSS4 oligonucleotide (500 ng) then end-filling with 250 μM each of dATP, dGTP, and dTTP, 50 μCi of 32P-dCTP and Klenow.