Words similar to datp

Example sentences for: datp

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[α- 32P]dATP and [α- 32P]UTP were purchased from Dupont NEN (Boston, MA).
The forward and reverse second round PCR primers were KK*ACAA*F2: 5'-AGTCAGGAATGGCTGCACC-3'and KK*ACAA*R: 5'-CAAATCAATC TTTGGGCAGG-3', respectively; the KK*ACAA*F2 primer was end-radiolabeled with 33P-dATP prior to PCR.
The oligo probes were radiolabeled using the Klenow fragment of DNA polymerase and 32P-dATP.
Small samples (2 μg) from each RNA fraction were reverse transcribed and radiolabeled (2 μg oligo dT, 1x First Strand buffer, 5 mM DTT, 75 U SuperscriptII [Life Technologies], 20 mM dNTP mix containing dATP, dGTP, dTTP and 100 mCi 33P-dCTP (ICN Radiochemicals).
The PCR product was digested with either the Eco RI or Xba I restriction enzyme, end-labeled with [αP 32]-dATP (ICN, Inc.) using the Klenow fragment of DNA polymerase I, and purified using a PCR clean-up kit (Qiagen).