Example sentences for: datp

How can you use “datp” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • [α- 32P]dATP and [α- 32P]UTP were purchased from Dupont NEN (Boston, MA).

  • RNA from an equivalent volume of each fraction was subjected to RT-PCR as just described, except the reactions were spiked with a small amount of radioactive dATP for quantitation.

  • The final reaction included 1X First Strand Buffer (Life Technologies), 5 mM DTT (Life Technologies), 1.5 mM dATP, dGTP and dTTP, 1.5 μl Superscript II Reverse Transcriptase (Life Technologies), and 10 μl of 33P-dCTP (ICN, specific activity 3,000 Ci/mmol).

  • Purified mRNAs, [α - 32P]dATP, dNTP mix and reverse transcriptase were incubated at 50°C for 25 min for the synthesis of radioactively labeled cDNA probes.

  • Radioactive products were made by 30 cycles of PCR amplification of 10-μl reaction volumes containing 10-30 ng of genomic DNA, 40 ng of each primer, 200 μM each of dATP, dTTP, dGTP, and dCTP, 1 μCi α- 32P-dCTP (3,000 Ci mmol -1) and 0.5 U Taq polymerase (Perkin Elmer).

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