Example sentences for: datp

How can you use “datp” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • For polyadenylate tailing at the 3' end of the cDNA, 5 μl tailing buffer (200 mmol/l potassium cacodylate [pH 7.2], 4 mmol/l CoCl 2 , 0.4 mmol/l DTT), 2 mmol/l dATP (Roche, Indianapolis, IN, USA), and 10 U terminal transferase (Roche) were added, and incubated at 37°C for 20 min, followed by heat inactivation at 65°C for 10 min.

  • Radioactive products were made by 30 cycles of PCR amplification of 10-μl reaction volumes containing 10-30 ng of genomic DNA, 40 ng of each primer, 200 μM each of dATP, dTTP, dGTP, and dCTP, 1 μCi α- 32P-dCTP (3,000 Ci mmol -1) and 0.5 U Taq polymerase (Perkin Elmer).

  • The following reagents were added: 6 μl of 5X first strand buffer, 3 μl 0.1 M DTT, 0.7 μl 50X dNTP (25 mM dATP, dCTP, dGTP and 10 mM dTTP), 3 μl 1 mM Cy3-dUTP or Cy5-dUTP and 1.7 μl Superscript™ II (200 U/μl).

  • 5 μCi of α-[ 35S] dATP (1270 Ci/mmole), and 1 U of AmpliTaq DNA polymerase (Perkin-Elmer, Foster City, CA).

  • Reverse transcription was performed in the presence of 500 μM each of dATP, dCTP, and dGTP, 200 μM 5-aminoallyl-dUTP (Sigma), 300 μM dTTP, 1 × first-strand buffer, 10 mM dithiothreitol, and 400 U Superscript II (Life Technologies) in 40-μl reactions at 42°C for 3 h to overnight.

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