Example sentences for: datp

How can you use “datp” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • In the first DNA-protein binding reaction, [ 32P]-labeled double stranded oligonucleotides were generated by first annealing BSS2 (50 ng) to the BSS4 oligonucleotide (500 ng) then end-filling with 250 μM each of dATP, dGTP, and dTTP, 50 μCi of 32P-dCTP and Klenow.

  • Two micrograms of total RNA was reverse transcribed with 32P-dATP, and the labeled fractions collected using ChromaSpin (human) columns.

  • Reverse transcription was performed in the presence of 500 μM each of dATP, dCTP, and dGTP, 200 μM 5-aminoallyl-dUTP (Sigma), 300 μM dTTP, 1 × first-strand buffer, 10 mM dithiothreitol, and 400 U Superscript II (Life Technologies) in 40-μl reactions at 42°C for 3 h to overnight.

  • Small samples (2 μg) from each RNA fraction were reverse transcribed and radiolabeled (2 μg oligo dT, 1x First Strand buffer, 5 mM DTT, 75 U SuperscriptII [Life Technologies], 20 mM dNTP mix containing dATP, dGTP, dTTP and 100 mCi 33P-dCTP (ICN Radiochemicals).

  • The PCR product was digested with either the Eco RI or Xba I restriction enzyme, end-labeled with [αP 32]-dATP (ICN, Inc.) using the Klenow fragment of DNA polymerase I, and purified using a PCR clean-up kit (Qiagen).


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