Words similar to datp

Example sentences for: datp

How can you use “datp” in a sentence? Here are some example sentences to help you improve your vocabulary:

For each sample, 50 μL solution containing approximately 5 units of Klenow large fragment (New England Biolabs, Beverly, MA), 3 μg of single stranded binding protein (US Biochemicals, Cleveland, OH), and 0.5 μM Cy3- or Cy5-labeled dATP, dCTP, dGTP or dUTP (Perkin Elmer) was pipeted onto the gel.
The purified 5 μg aRNA was labeled with Cy3 or Cy5 by reverse transcription in a solution containing 10 μg of random hexamer, 4 μl first-strand reaction buffer, 2 μl 10 mM dithiothreitol (DTT; Gibco-BRL), 1 μl of 2 mM dATP, dGTP, dTTP and 1 mM dCTP, 1 μl SUPERase-in, (Amtion) and 200 u Superscript II reverse transcriptase (Gibco-BRL).
5 μCi of α-[ 35S] dATP (1270 Ci/mmole), and 1 U of AmpliTaq DNA polymerase (Perkin-Elmer, Foster City, CA).
Reverse transcription was performed in the presence of 500 μM each of dATP, dCTP, and dGTP, 200 μM 5-aminoallyl-dUTP (Sigma), 300 μM dTTP, 1 × first-strand buffer, 10 mM dithiothreitol, and 400 U Superscript II (Life Technologies) in 40-μl reactions at 42°C for 3 h to overnight.
Probes were 3'-end labeled with 35S or 33P-dATP using a terminal transferase reaction and hybridization carried out [ 26 ] . Autoradiograms were generated by dipping slides in NTB2 nuclear track emulsion and storing in the dark at 4°C.