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Example sentences for: dapk

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The primers used for amplification of methylated and unmethylated promoters of genes RARB , TIMP3 , CDKN2A , p14 ARF , MGMT , DAPK , CDH1 , GSTP1 , APC promoter 1A, RB1 , MLH1 , TP73 , BRCA1 , FHIT , and HIC1 have been described previously http://pathology2.jhu.edu/pancreas/prim0425.htm#MSP; [ 32 47 48 49 ] . Methylated (MF and MR) and unmethylated (UF and UR) primers for RASSF1A promoter were designed according to Herman et al [ 46 ] and as described earlier [ 50 ] . PCR products were run on 2% agarose gels and visualized after ethidium bromide staining.
Of the six cell lines with down regulated expression, 2 had promoter methylation and 4 had unmethylated promoter of the DAPK gene.
CDH1, DAPK, RARB and HIC1 gene promoters are frequently methylated in CC
The primers used for CDH1 , MGMT , MLH1 , and RARB have been described earlier [ 50 ] . The gene primers used for DAPK and RASSF1A and their positions in respective cDNAs were:
Although the patterns are similar, primary tumors had a higher frequency of methylation of CDH1 and DAPK genes as compared to cell lines, while RARB , HIC1 , RASSF1A , MGMT , and TP73 genes had higher prevalence in cell lines as compared to primary tumors (Table 1).