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Example sentences for: dapk
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Therefore, our data further suggest a role for DAPK in CC tumorigenesis.
To examine the biological role of promoter methylation in CC, we assessed the levels of mRNA by semi-quantitative RT-PCR in eight cell lines and compared with the respective control values obtained from the averages calculated from 2 normal cervix after normalization against ACTB . This analysis was performed on 7 genes ( CDH1 , DAPK , RARB , HIC1 , MGMT , RASSF1A , and MLH1 ) that exhibited methylation in one or more cell lines and primary tumors (Fig.
The CDH1 , DAPK , RARB , and HIC1 gene promoters were frequently methylated.
The primers used for methylated and unmethylated-specific PCR for genes RARB , TIMP3 , CDKN2A , p14 ARF , MGMT , DAPK , CDH1 , GSTP1 , APC promoter 1A, RB1 , MLH1 , TP73 , BRCA1 , FHIT , and HIC1 have been described previously http://pathology2.jhu.edu/pancreas/prim0425.htm#MSP; [ 34 35 36 37 ] . For additional genes, we designed the following gene-specific primers for methylated (MF and MR) and unmethylated (UF and UR) sequences according to Herman et al [ 33 ] :
Dong and co-workers also previously showed promoter methylation in 51% of CC [ 13 ] . DAPK is a positive mediator of the programmed cell death induced by gamma-interferon [ 21 ] . Loss of DAPK expression has been shown to occur in a number of human malignancies, primarily by promoter hypermethylation [ 11 ] . It has also been shown that loss of DAPK gene expression was associated with aggressive and metastatic phenotype in many tumor types [ 22 ] . In the present study, in addition to showing high frequency of promoter methylation of DAPK gene, we found a complete lack of expression in two of the methylated cell lines and four of 6 unmethylated cell lines.
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