Words similar to dadle
Example sentences for: dadle
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A rat monoclonal anti-idiotypic antibody (anti-Id mAb; 8 mg/ml;), generated against an anti- D-Ala 2-D-Leu 5-Enkephalin (DADLE) antibody and displaying affinity for δ-opioid receptors ( 10, 12), was used as the primary antibody.
Third, in rat spinal cord ( 12), we found that preincubation of fixed tissue with specific δ-opioid ligands (DADLE, Met-enkephalin, dermenkephalin and naltrindole at 10 -9M) totally prevented the antibody labeling, showing a good competition between ligands and the anti-Id mAb.
First, the anti-Id mAb raised against an anti DADLE ( 10, 11) totally inhibited the δ-agonist ligand/anti-ligand interaction, showing a good anti-idiotypic activity.
At concentrations of 10 μM, neither DSLET, DADLE, nor DPDPE were found to increase the tyrosine phosphorylation of the IR or the PDGFR, whereas insulin (100 ng/ml) or PDGF (50 ng/ml) induced significant tyrosine phosphorylation of these sites (Figure 8).
In vitro studies carried out on NG108-15, a neurohybrid cell expressing δ-opioid receptors, showed internalization after the application of opioid agonists in bath medium, like DADLE or etorphin ( 9, 31).