Words similar to cumulus
Example sentences for: cumulus
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Aspirated oocytes that had an evenly granulated cytoplasm and were surrounded by at least two uniform layers of compact cumulus cells were selected and washed three times in Hepes-buffered synthetic oviductal fluid medium [ 58].
The blastocyst formation rate in our study is similar to results from the recent reports in which porcine adult somatic (cumulus) cells (5-10%) [ 29, 51] or fetal fibroblasts (1-16%) [ 11, 29, 51, 52, 53, 54, 55, 56, 57] were transferred into enucleated M II oocytes matured in media supplemented with pFF.
Cumulus cells were removed by treating eggs for 3 min with 1 mg/ml hyaluronidase (Sigma, St. Louis, MO) in M199; eggs were washed through four 50-ml drops of M199 medium covered with mineral oil using a pulled , heat-polished, Pasteur pipette (employed in all experiments).
from selection at E 0200 h through ovulation at E 0200 h the next cycle), the membrana, but not the cumulus and periantral GC, express high levels of BMP-2 mRNA (Fig.
Unlike somatic ubiquitous hyaluronidases that are active only at acidic pH [ 8 ] , SPAM1 has hyaluronidase activity at both neutral and acidic pHs, which arise from two different regions within the hyaluronidase domain [ 4 ] . The neutral enzyme activity, which is predominant in insoluble membrane-bound SPAM1, is necessary for sperm to penetrate the hyaluronic acid (HA)-rich extracellular matrix of the cumulus cells surrounding the oocyte.