Words similar to ctg

Example sentences for: ctg

How can you use “ctg” in a sentence? Here are some example sentences to help you improve your vocabulary:

Following extraction, we first amplified the picornavirus RNA using primers OL26 (5'-GCA CTT CTG TTT CCC C-3') [ 33 ] and OL27 (5'-CGG ACA CCC AAA GTA G-3') [ 33 ] using the Invitrogen SuperScript One-Step RT-PCR with PLATINUM Taq Kit (Invitrogen Corp., Carlsbad, California).
Conversely, detecting negative selection at a cta or ctg codon is difficult.
The gap between ctg16020 and ctg16021 corresponds to gap 1 (see additional data file 1, blau_map.pdf).
Binding reactions were carried out at room temperature for 30 min in a total volume of 15 μl and contained 2-5 μg of nuclear extracts, 5 μl of 5X gel shift binding buffer (20% glycerol, 5 mM MgCl 2 , 2.5 mM EDTA, 2.5 mM DTT, 250 mM NaCl, 50 mM Tris-HCl, pH 7.5), 2 μg poly (dI-dC) and 3 × 10 4cpm/μl of [ 32P] labeled CREB (5'-AGA GAT TGC CTG ACG TCA GAG AGC TAG-3'), NF-κB (5'-AGT TGA GGG GAC TTT CCC AGG C-3') (Promega Gel Shift Assay Systems, Madison, WI, USA) or CCAAT/enhancer binding protein (C/EBP) (5'-TGC AGA TTG CGC AAT CTG CA-3') (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) oligonucleotides.
Point mutations were introduced into Rlk-GFP by a PCR based strategy [ 53] with oligonucleotides encoding the required mutations (Mutated residues are in bold): Y420F (GGAC GAT GAA T TC ATC AGT TCT TCT G), Y91F (GTC AAG GCT CTT T T T GAC TTC CTG CC).