Words similar to cryostat
Example sentences for: cryostat
How can you use “cryostat” in a sentence? Here are some example sentences to help you improve your vocabulary:
For frozen tissues, 5 μm cryostat sections (Leica Microsystems, Heidelberger, Germany) were briefly fixed in acetone.
For fluorescent detection of RNA hybridization and antibody labeling, embryos were first treated for RNA hybridization and then sectioned using a cryostat microtome.
For preparation of protein lysates from placental tissue and ovarian cancer for western blot analysis, including control Cdk2 and normal rabbit immunoglobulin immunoprecipitates (IP), 150 cryostat tissue sections (7μm) were collected into microcentrifuge tubes and lysed by adding ice-cold lysis buffer (20 mM Tris pH 7.5, 200 mM NaCl, 0.25% Nonidet P-40; 400 μl/100 mg of tissue sections) containing 1 mM sodium orthovanadate, 10 mM sodium fluoride, and 1 mM phenylmethylsulfonyl fluoride.
Sixteen micron thick horizontal sections of spinal cord or sciatic nerve through the crush injury were subsequently prepared using a cryostat and thaw-mounted onto uncoated 35 mm plastic culture dishes (Fisher Scientific, Cincinnati, OH).
Serial coronal cryostat sections (20 μm thick) were hybridized overnight at 55°C and washed at 57°C.