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Example sentences for: cruz
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Immunohistochemical staining was done using rabbit polyclonal antibodies raised against synthetic peptides that correspond to regions in the mature forms of TGF-β 1 , TGF-β 2 and TGF-β 3 : anti-TGF-β 1 -LC and anti-TGF-β 1 -CC [ 19], anti-TGF-β 2 (sc-90; Santa Cruz Biotechnologies Inc, Santa Cruz, CA, USA), and anti-50-60-β 3 -LC [ 20], respectively.
These days Santa Cruz is best known for the Aeroporto de Santa Cataria (international airport) that serves Funchal and all of Madeira.
The blocking peptide sc-6820 P (Santa Cruz Biotechnology) for N-19 ERβ antibody was mixed with the ERβ antibody at a 10:1 weight ratio, and incubated overnight at 4°C then 2 h at room temperature before the peptide-absorbed antibody was utilized for immunohistochemistry (PA/ERβ, Figure 2H) and western blot controls (PA/ERβ, Figure 6B).
Anti-Fyn (FYN3) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA); anti-phosphotyrosine (4G10) from Upstate Biotechnologies (Lake Placid, New York).
The eIF-2 was precipitated with a polyclonal antibody against human eIF-2α (C-20) (Santa Cruz Biotech) in the presence of protein G-Sepharose (Amersham) for 1 h at 10°C, and then processed as described previously [ 21 ] . The immunoprecipitates were subjected to SDS-PAGE followed by autoradiography to examine eIF-2 phosphorylation.