Example sentences for: cross-linked

How can you use “cross-linked” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The amplimers were UV cross-linked and hybridized with Rapid-Hyb buffer (Amersham) at 42°C using γ- 32P ATP end-labeled oligonucleotides (Table 1, G3PDH probe from Clontech) as probes.

  • A sample of 0.8 μl of a 1:300 dilution of the probe was spotted on a positively charged nylon membrane (Roche) with a 96-well pin tool (V&P Scientific) and then cross-linked to the membrane by UV irradiation.

  • After printing, DNA was cross-linked to the slides by UV irradiation with a Stratalinker UV Crosslinker (Stratagene, La Jolla, CA) and stored in a vacuum chamber until use.

  • The RNA was cross-linked to the membrane by exposure to UV light and hybridized with specific probes labeled with 32P-dCTP (Perkin Elmer Life Sciences, Boston, MA) using the Prime-It random prime labeling kit (Stratagene, La Jolla, CA).

  • Total RNA (20 μg) was isolated from various cells and ran on a 1% formaldehyde-agarose gel overnight at 75 V, transferred onto a 0.2 μm nitrocellulose membrane (Millipore Inc.), UV cross-linked, and hybridized overnight at 42°C with 32P-end-labeled 40 mer oligo probes including p21/Waf1, C-myc, Pro-thymosin, Actin, Tat, and Ubiquitin (Loftstrand, Gaithersburg, Md.).


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