Words similar to cross-linked
Example sentences for: cross-linked
How can you use “cross-linked” in a sentence? Here are some example sentences to help you improve your vocabulary:
In the study reported here, we systematically compared the performance of unmodified 70-mer oligonucleotides to traditional PCR amplicons, both probe types printed and UV cross-linked onto glass slides coated with primary amine groups.
Total RNA (20 μg) was isolated from various cells and ran on a 1% formaldehyde-agarose gel overnight at 75 V, transferred onto a 0.2 μm nitrocellulose membrane (Millipore Inc.), UV cross-linked, and hybridized overnight at 42°C with 32P-end-labeled 40 mer oligo probes including p21/Waf1, C-myc, Pro-thymosin, Actin, Tat, and Ubiquitin (Loftstrand, Gaithersburg, Md.).
The amplimers were UV cross-linked and hybridized with Rapid-Hyb buffer (Amersham) at 42°C using γ- 32P ATP end-labeled oligonucleotides (Table 1, G3PDH probe from Clontech) as probes.
The membrane was cross-linked with UV light (Stratalinker 1800, Stratagene, La Jolla, CA, USA) and stored for analysis by hybridization.
Buthoid venom has been reported to strongly affect a wide variety of vertebrate and invertebrate organisms [ 4 ] and its toxicity is attributed to the presence of a large variety of basic polypeptides cross-linked by 3-4 disulfide bridges [ 1 4 ] . Based on their molecular size and pharmacological activity, these toxins are classified into two main groups.