Example sentences for: cross-linked

How can you use “cross-linked” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Total RNA (20 μg) was isolated from various cells and ran on a 1% formaldehyde-agarose gel overnight at 75 V, transferred onto a 0.2 μm nitrocellulose membrane (Millipore Inc.), UV cross-linked, and hybridized overnight at 42°C with 32P-end-labeled 40 mer oligo probes including p21/Waf1, C-myc, Pro-thymosin, Actin, Tat, and Ubiquitin (Loftstrand, Gaithersburg, Md.).

  • The RNA was cross-linked to the membrane by exposure to UV light and hybridized with specific probes labeled with 32P-dCTP (Perkin Elmer Life Sciences, Boston, MA) using the Prime-It random prime labeling kit (Stratagene, La Jolla, CA).

  • Clones were printed in duplicate from 50% DMSO onto SuperAmine slides (Telechem International) as described previously and cross-linked at 90 mJ using a Stratalinker (Stratagene).

  • After printing, DNA was cross-linked to the slides by UV irradiation with a Stratalinker UV Crosslinker (Stratagene, La Jolla, CA) and stored in a vacuum chamber until use.

  • Buthoid venom has been reported to strongly affect a wide variety of vertebrate and invertebrate organisms [ 4 ] and its toxicity is attributed to the presence of a large variety of basic polypeptides cross-linked by 3-4 disulfide bridges [ 1 4 ] . Based on their molecular size and pharmacological activity, these toxins are classified into two main groups.


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