Example sentences for: cross-linked

How can you use “cross-linked” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The amplimers were UV cross-linked and hybridized with Rapid-Hyb buffer (Amersham) at 42°C using γ- 32P ATP end-labeled oligonucleotides (Table 1, G3PDH probe from Clontech) as probes.

  • Total RNA (20 μg) was isolated from various cells and ran on a 1% formaldehyde-agarose gel overnight at 75 V, transferred onto a 0.2 μm nitrocellulose membrane (Millipore Inc.), UV cross-linked, and hybridized overnight at 42°C with 32P-end-labeled 40 mer oligo probes including p21/Waf1, C-myc, Pro-thymosin, Actin, Tat, and Ubiquitin (Loftstrand, Gaithersburg, Md.).

  • [ 52 ] air dried and then UV cross-linked using a Stratagene crosslinker.

  • The antisera were affinity-purified and tested for specificity by immunoprecipitation of cross-linked complexes of cultured cells transfected with receptor complementary deoxyribonucleic acids (cDNAs) [ 11].

  • Following DNA spotting and neutralization, the membranes were washed by placing on filter paper saturated in 2X SSC for 2 min, removed from filter paper, UV cross-linked and stored at 4°C.


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