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Example sentences for: crassus
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We have begun to examine this data-set for TAS-adjacent sequence motifs that potentially define the binding-sites and/or cut-sites of single-TAS MAC ends, as Klobutcher et al . [ 38 ] have done for E. crassus . We can then test the prediction that multi-TAS regions bear either weak binding- or cut-sites.
Furthermore, the in vitro exconjugant enzyme of Euplotes crassus can cap non-telomeric DNA, aided by Chromosome Healing Factor [ 42 ] . That Euplotes is more closely related to Oxytricha than to Tetrahymena tentatively supports our assumption that telomerase can heal ends well after the binder/cutter has acted.
Thus, in Tetrahymena and Paramecium telomeres are added at a heterogeneous collection of clustered sites, which we here refer to as "multi-TAS regions," whereas in E. crassus only one TAS is used for each MAC-destined end ("single TAS regions").
The in vitro properties of telomerase are consistent with its known role in creation of MAC telomeres in Tetrahymena [ 41 ] , as well as, presumably, in all other ciliates [ 9 ] . Telomerases from exconjugants of Tetrahymena and Euplotes have been extensively studied in vitro . The E. crassus enzyme can only act on oligomers that pair with telomerase template RNA at their 3' terminus, unless assisted by a dissociable factor (chromosome healing factor), which contributes extra mass to the enzyme in exconjugants [ 42 ] . Telomerase, assisted by this factor, still requires a block of dGs or telomere repeats internal to the 3' primer end [ 43 ] . In contrast, the Tetrahymena exconjugant enzyme can add to a primer completely devoid of telomere repeats in special reaction conditions, and shows no evidence of increased mass beyond that of the vegetative enzyme [ 44 ] .
. In stark contrast, no heterogeneity of TAS positions has been reported for E. crassus MAC chromosomes, although recently Möllenbeck and Klobutcher [ 52 ] report multiple TASs at the end of a MIC-limited "spacer" segment.
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