Words similar to cpm
Example sentences for: cpm
How can you use “cpm” in a sentence? Here are some example sentences to help you improve your vocabulary:
The cells were then harvested on filter papers using a harvester, filter papers were dried and were then transferred to liquid scintillation vials and [ 3H] thymidine uptake were quantified as counts per minute (CPM) using a liquid scintillation counter.
Northern blot analysis was carried out using double-stranded DNA probes labeled to a specific activity of 10 8- 10 9cpm/μg with α-[ 32P] CTP using T7 DNA polymerase and random primers.
Twenty micrograms of total RNAs from rabbit tissues, preglomerular vessels, cultured cells were incubated at 45°C overnight in hybridization buffer with 1 × 10 5cpm labeled riboprobe.
The blots were pre-hybridized in a solution containing 6-strength SSC (150 mM NaCl, 15 mM Na-citrate, pH 7.0), 0.5% SDS, 50% formamide, and 100 mg/ml denatured sperm DNA at 42°C for 2-4 h, and were hybridized overnight in the same buffer containing approximately 10 6cpm/ml of radiolabeled cDNA probe.
Nytran membranes with immobilized cDNAs for the BlueScript vector, human SP-A and human α-actin were prehybridized in 1 ml of buffer (50% formamide, 5 X SSC, 5 X Denhardt's solution, 100 μg/ml denatured herring sperm, 0.1% SDS) for 4 hours at 45°C and then hybridized to the labeled RNAs (6 × 10 6cpm in the presence of 500 units of RNasin) in duplicate for an additional 60 hours.
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