Example sentences for: cpm

How can you use “cpm” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Binding reactions were carried out at room temperature for 30 min in a total volume of 15 μl and contained 2-5 μg of nuclear extracts, 5 μl of 5X gel shift binding buffer (20% glycerol, 5 mM MgCl 2 , 2.5 mM EDTA, 2.5 mM DTT, 250 mM NaCl, 50 mM Tris-HCl, pH 7.5), 2 μg poly (dI-dC) and 3 × 10 4cpm/μl of [ 32P] labeled CREB (5'-AGA GAT TGC CTG ACG TCA GAG AGC TAG-3'), NF-κB (5'-AGT TGA GGG GAC TTT CCC AGG C-3') (Promega Gel Shift Assay Systems, Madison, WI, USA) or CCAAT/enhancer binding protein (C/EBP) (5'-TGC AGA TTG CGC AAT CTG CA-3') (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) oligonucleotides.

  • Human fibrinogen was radiolabeled with 125I (Amersham, Chicago, IL) using Iodobeads (Pierce, Rockford, IL) as described [ 48 ] . Different numbers of bacteria were incubated with 20,000 cpm of 125I labeled fibrinogen for 60 min at 37°C.

  • 3 × 10 5cpm of labeled probe were hybridized with 10 μg of total RNA for 16 h at 56°C.

  • For each reaction, 5 × 10 3cpm of labeled probe was incubated with 5 μg of crude nuclear proteins from Sertoli cells and 1.0 μg of poly dI:dC in a buffer containing 5 mM Hepes pH 7.9, 26 % glycerol, 0.2 mM EDTA, 0.5 mM DTT, 0.5 mM PMSF, 150 mM KCl and 5 mM MgCl at room temperature for 15 min.

  • Twenty micrograms of total RNAs from rabbit tissues, preglomerular vessels, cultured cells were incubated at 45°C overnight in hybridization buffer with 1 × 10 5cpm labeled riboprobe.


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