Words similar to cpm
Example sentences for: cpm
How can you use “cpm” in a sentence? Here are some example sentences to help you improve your vocabulary:
[ 38 ] Briefly, radiolabeled phosphorylase a was synthesized by taking phosphorylase b (Sigma), at a concentration of 10 mg/ml, and incubating with 0.2 mg/ml phosphorylase kinase (Sigma) for 1.5 hours at 30°C in kinase reaction buffer (100 mM Tris-HCl; 100 mM sodium glycerol-1-phosphate, pH 8.2; 0.1 mM CaCl 2 , 10 mM magnesium acetate, and 0.2 mM [g- 32P]ATP {10 6cpm/nmol}).
Nytran membranes with immobilized cDNAs for the BlueScript vector, human SP-A and human α-actin were prehybridized in 1 ml of buffer (50% formamide, 5 X SSC, 5 X Denhardt's solution, 100 μg/ml denatured herring sperm, 0.1% SDS) for 4 hours at 45°C and then hybridized to the labeled RNAs (6 × 10 6cpm in the presence of 500 units of RNasin) in duplicate for an additional 60 hours.
Binding reactions were carried out at room temperature for 30 min in a total volume of 15 μl and contained 2-5 μg of nuclear extracts, 5 μl of 5X gel shift binding buffer (20% glycerol, 5 mM MgCl 2 , 2.5 mM EDTA, 2.5 mM DTT, 250 mM NaCl, 50 mM Tris-HCl, pH 7.5), 2 μg poly (dI-dC) and 3 × 10 4cpm/μl of [ 32P] labeled CREB (5'-AGA GAT TGC CTG ACG TCA GAG AGC TAG-3'), NF-κB (5'-AGT TGA GGG GAC TTT CCC AGG C-3') (Promega Gel Shift Assay Systems, Madison, WI, USA) or CCAAT/enhancer binding protein (C/EBP) (5'-TGC AGA TTG CGC AAT CTG CA-3') (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) oligonucleotides.
Northern blot analysis was carried out using double-stranded DNA probes labeled to a specific activity of 10 8- 10 9cpm/μg with α-[ 32P] CTP using T7 DNA polymerase and random primers.
Has Salon 's advertising CPM actually sunk by two-thirds?
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