Example sentences for: cpm

How can you use “cpm” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The cells were then harvested on filter papers using a harvester, filter papers were dried and were then transferred to liquid scintillation vials and [ 3H] thymidine uptake were quantified as counts per minute (CPM) using a liquid scintillation counter.

  • Northern blot analysis was carried out using double-stranded DNA probes labeled to a specific activity of 10 8- 10 9cpm/μg with α-[ 32P] CTP using T7 DNA polymerase and random primers.

  • Twenty micrograms of total RNAs from rabbit tissues, preglomerular vessels, cultured cells were incubated at 45°C overnight in hybridization buffer with 1 × 10 5cpm labeled riboprobe.

  • The blots were pre-hybridized in a solution containing 6-strength SSC (150 mM NaCl, 15 mM Na-citrate, pH 7.0), 0.5% SDS, 50% formamide, and 100 mg/ml denatured sperm DNA at 42°C for 2-4 h, and were hybridized overnight in the same buffer containing approximately 10 6cpm/ml of radiolabeled cDNA probe.

  • Nytran membranes with immobilized cDNAs for the BlueScript vector, human SP-A and human α-actin were prehybridized in 1 ml of buffer (50% formamide, 5 X SSC, 5 X Denhardt's solution, 100 μg/ml denatured herring sperm, 0.1% SDS) for 4 hours at 45°C and then hybridized to the labeled RNAs (6 × 10 6cpm in the presence of 500 units of RNasin) in duplicate for an additional 60 hours.


How many words do you know? Try our free vocabulary size test!


Search

Search for example sentences

Loading Loading...
Quantcast