Words similar to cpm
Example sentences for: cpm
How can you use “cpm” in a sentence? Here are some example sentences to help you improve your vocabulary:
Hybridization was carried out with the 35S-labeled RNA probe (4-6 × 10 6cpm/ml) in a solution containing 50% (vol/vol) deionized formamide, 0.3 M NaCl, 10 mM Tris (pH 8.2), 1 mM EDTA, 0.05% yeast tRNA, 10 mM dithiothreitol, 1 × Denhardt's solution and 10% dextran sulfate.
Filters were hybridized at 65°C overnight with either labeled Drosophila body (minus head) or labeled Drosophila brain cDNA at 1 × 10 6cpm ml -1in Church and Gilbert buffer (7% SDS, 1 mM EDTA, 500 mM Na 2 HPO 4 , and pH to 7.2 with H 3 PO 4 [ 44]).
Nytran membranes with immobilized cDNAs for the BlueScript vector, human SP-A and human α-actin were prehybridized in 1 ml of buffer (50% formamide, 5 X SSC, 5 X Denhardt's solution, 100 μg/ml denatured herring sperm, 0.1% SDS) for 4 hours at 45°C and then hybridized to the labeled RNAs (6 × 10 6cpm in the presence of 500 units of RNasin) in duplicate for an additional 60 hours.
The cells were then harvested on filter papers using a harvester, filter papers were dried and were then transferred to liquid scintillation vials and [ 3H] thymidine uptake were quantified as counts per minute (CPM) using a liquid scintillation counter.
o for each fluorophore, where B is the cpm of [ 125I] SP specifically bound in the presence of non-radioactive SP and B o is the cpm of [ 125I] SP specifically bound in the presence of 100 pM fluorescently labeled SP.
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