Words similar to coverslips
Example sentences for: coverslips
How can you use “coverslips” in a sentence? Here are some example sentences to help you improve your vocabulary:
The coverslips were removed for fluorescence microscopic observation immediately before collecting cells for flow cytometric analysis of DNA content and GFP expression.
Coverslips were washed 3× with PBS and stored at room temperature before staining.
7% formaldehyde at -20°C for 20 minutes, then washed twice with 1 × PBS; the coverslips where then incubated with a 1:200 dilution of rabbit anti-cyclin D 1 antibody (Santa Cruz Biotechnology) or control rabbit sera (Biogenex) for 2 hours at room temperature followed by three washes with 1 × PBS; the coverslips were then incubated with a 1:250 dilution of rhodamine-labeled donkey-anti-rabbit anti-sera (Chemicon) for 45 minutes to detect the primary antibody; following three final washes with 1 × PBS, the coverslips were mounted on glass slides using ProLong Antifade (Molecular Probes) for fluorescent detection of GFP and cyclin D 1 on an Olympus IX70 microscope at 100 × magnification.
Cells grown on coverslips were treated with H 2 O 2 for various periods of time as indicated in figure legends, fixed for 10 minutes with 3.7% formaldehyde (prepared in PBS), stained with 1 μg/ml bisbenzimide solution (Hoechst 33258, Sigma, St. Louis, MO) for 10 minutes at room temperature, mounted with GEL/MOUNT, then observed under a fluorescent microscope as described in the previous section.
Cells grown on coverslips were washed briefly in a phosphate-buffered saline (PBS) solution (pH 7.4, 137 mM NaCl, 2.7 mM KCl, 4.3 mM Na 2 HPO 4 , 1.4 mM KH 2 PO 4 ) and then either fixed in 100% methanol at -20°C for 1 minute or at room temperature in a 4% formaldehyde (PFA) solution freshly made from paraformaldehyde (Electron Microscopy Sciences, Ft.
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