Example sentences for: counter-selectable

How can you use “counter-selectable” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Approximately the same yield of gene-positive clones had been previously observed during TAR cloning of this genomic region with vectors lacking ARS and a counter-selectable marker [ 7 15 ] . No transgene-positive clones were found when 5-FOA selection was omitted (Table 3), indicating that the new TAR cloning method is highly selective and efficient for isolation of multicopy genes.

  • Vectors with a counter-selectable marker at the end of a targeting hook are not efficient for gene isolation from complex genomes

  • The first strategy exploited a vector with a counter-selectable marker placed at the end of one of the targeting hooks.

  • We previously demonstrated that Alu -containing TAR cloning vectors that lack ARS elements are efficient for selective isolation of human DNA from human-rodent monochromosomal hybrid cell lines [ 27 ] . Our new counter-selectable system allows construction of representative chromosome-specific libraries.

  • That observation led to the development of a system for investigating the expansion of trinucleotide repeats in yeast in which the promoter is combined with the URA3 gene, a widely used counter-selectable marker [ 19 23 ] . In our work, we took advantage of the specific spacing requirements of the ADH1 promoter to develop a novel TAR cloning procedure that allows isolation of genomic regions that lack ARS elements.


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