Words similar to counter-selectable
Example sentences for: counter-selectable
How can you use “counter-selectable” in a sentence? Here are some example sentences to help you improve your vocabulary:
Isolation of specific chromosomal regions with a TAR vector containing ARS and a counter-selectable marker
We previously demonstrated that Alu -containing TAR cloning vectors that lack ARS elements are efficient for selective isolation of human DNA from human-rodent monochromosomal hybrid cell lines [ 27 ] . Our new counter-selectable system allows construction of representative chromosome-specific libraries.
The first strategy exploited a vector with a counter-selectable marker placed at the end of one of the targeting hooks.
That observation led to the development of a system for investigating the expansion of trinucleotide repeats in yeast in which the promoter is combined with the URA3 gene, a widely used counter-selectable marker [ 19 23 ] . In our work, we took advantage of the specific spacing requirements of the ADH1 promoter to develop a novel TAR cloning procedure that allows isolation of genomic regions that lack ARS elements.
These results demonstrate that TAR cloning vectors with a counter-selectable marker at the end of one of the hooks are inefficient for cloning the genes from complex mammalian genomes.