Words similar to cotyledons
Example sentences for: cotyledons
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27 to 0.33 M) concentrations of glucose or sucrose has been shown to severely inhibit development of Arabidopsis seedlings [ 33 46 47 48 49 50 55 ] . Most seedlings grown on high-sugar media lack true leaves and have cotyledons that have undergone little expansion, even after two to three weeks of growth.
In addition, the facts that they are rapidly growing and metabolizing, were shown to produce ample quantities of subunit A transcript, and possess several organs including roots, hypocotyls, and cotyledons made them an attractive choice for analysis.
In contrast, dark-grown seedlings undergo skotomorphogenesis (or etiolation), typified by elongated hypocotols and closed cotyledons [ 1 ] . COP1 was first identified through genetic screens as a negative regulator of light regulated development in Arabidopsis [ 2 ] . Arabidopsis cop1 mutant seedlings are constitutively photomorphogenic even when grown in the dark, and the severe cop1 mutants cause lethality in the late seedling stage, indicating that COP1 is essential for plant development [ 2 3 ] . Arabidopsis COP1 (Arabidopsis thaliana COP1, AtCOP1) is essential for the proteasome-dependent degradation of two transcription factors, HY5 and HYH [ 4 5 ] . These two homologous bZIP-type transcription factors directly interact with AtCOP1 and are capable of binding to light-responsive promoters to activate the transcription of many target genes [ 5 6 ] . Genome wide micro-array analysis shows that AtCOP1 regulates most, if not all, of the light-responsive genes under various light conditions [ 7 8 ] , substantiating the notion that AtCOP1 functions as a crucial developmental switch through targeting key transcription factors for degradation, thereby controlling the light-responsive gene expression and photomorphogenic development.
For SSH experiments, 4-10 leaves were harvested, avoiding the most immature leaves, cotyledons, and the first two true leaves.
5. Plants in which GFP-COP1 expression was restricted to roots and cotyledons were regarded as silenced, whereas plants with GFP-COP1 visible beyond the cotyledons were considered active.
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