Words similar to cotransfected

Example sentences for: cotransfected

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CV-1 cells were cotransfected with a reporter plasmid responsive to androgen (ARE-TATA-luciferase) in the presence or absence of AR and constitutively active Stat3C (Fig.
Interestingly, when all three constructs - SRF, Elk-1, and p35-C/EBPβ-are cotransfected with the reporter construct, there is an average 260-fold increase in CAT activity in the presence of activated Ras.
The transfection efficiency was normalized by determining β-galactosidase activity in the lysates from the cotransfected pLacZ plasmid.
To determine if this activation of p53 transcriptional activity required the GSK3β phosphorylation site at serine 33, wtp53 with serine to alanine mutations at either positions 33 or 37 were cotransfected with GSK3β.
Primary cultures were prepared from hind limbs of day 18 embryos (E18) as described previously [ 23 ] . cDNAs of interest and a separate expression vector encoding the T-cell membrane antigen CD8 were subcloned into the mammalian expression vector pSG5 (Stratagene, CA) and were mixed and cotransfected with the polyamine LT-1 (Panvera, WI).