Words similar to cotransfected

Example sentences for: cotransfected

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The transfection efficiency was normalized by determining β-galactosidase activity in the lysates from the cotransfected pLacZ plasmid.
However, when myotubes were cotransfected with fs-α 1S M701I and α 1S Δ1-700, each in a separate pSG5 vector, there was a robust recovery of Ca 2+transients in 5 of 5 cells.
Interestingly, when all three constructs - SRF, Elk-1, and p35-C/EBPβ-are cotransfected with the reporter construct, there is an average 260-fold increase in CAT activity in the presence of activated Ras.
Triplicate dishes of SCC25 cells were transiently transfected with 5 μg luciferase reporter construct containing a heterologous promoter containing a consensus AP-1 site 5'-TGACTCA-3' [ 11 ] . 2 μg of wild type or S77A mutant RARα expression vector was cotransfected to determine the effects of RARα phosphorylation on AP-1 promoter activity.
Primary cultures were prepared from hind limbs of day 18 embryos (E18) as described previously [ 23 ] . cDNAs of interest and a separate expression vector encoding the T-cell membrane antigen CD8 were subcloned into the mammalian expression vector pSG5 (Stratagene, CA) and were mixed and cotransfected with the polyamine LT-1 (Panvera, WI).