Words similar to cos-
Example sentences for: cos-
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Transient expression of the Src*/Cas(SD) in Cos-7 cells followed by analysis of total cell lysates using anti-phosphotyrosine antibodies demonstrated that while the attenuated Src kinase domain alone did not increase total cellular tyrosine phosphorylated proteins as expected, expression of the Src*/Cas(SD) fusion resulted in tyrosine phosphorylation of a protein of the expected size of the chimera, as well as other proteins (Fig.
To determine if the tyrosine phosphorylated chimera could compete with endogenous Cas for binding to endogenous c-crk, we transfected either the isolated attenuated Src kinase domain as control, or the tyrosine phosphorylated Cas substrate domain (Src*/Cas(SD)) into Cos-7 cells, and immunoprecipitated endogenous c-crk (Fig.
In this report, we questioned whether the EGFR acts as a surrogate scaffold during ERK activation through the δ-OR, as it appears to do for several other GPCRs [ 7 ] . To act as a surrogate-binding site for complex formation, the literature states that this protein must first become tyrosine-phosphorylated [ 7 ] . Tyrosine phosphorylation of the EGFR is apparent after agonist stimulation of the α 2 -AR (COS-7), β 2 -AR (COS-7), insulin-like growth factor (IGF-1; COS-7), substance P, and the lysophosphatidic acid receptor (LPAR) [ 7 ] . In addition, each of the aforementioned ligands also increases ERK phosphorylation when the proper receptor is expressed in these cell lines.
RGS3T is comprised primarily by the consensus RGS domain and was originally reported to decrease the growth rate of yeast and to inhibit IL-8 receptor mediated activation of MAP kinase by a factor of 3-10 fold as compared to RGS3 [ 24 ] and was subsequently reported to be a normally expressed variant of RGS3 [ 37 ] . Therefore, we compared it to RGS3 for attenuation of GnRH-stimulated IP 3 production in COS-1 cells (Fig.
COS-7 cells were harvested in PBS containing 0.1 mM sodium vanadate and collected by low speed centrifugation.
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