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Example sentences for: cos-

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RP L4/L1 transcripts, which were not significantly affected by NGF, are selectively upregulated in PC12 cells prior to 5 aza cytosine-induced death and over-expression of this gene in COS-7 cells induces apoptosis [ 39 ] . Over-expression of L7, L13A, S29 have been also reported to induce apoptosis [ 40 41 ] . In the present study, NGF promoted little if any change in expression of these RP transcripts.
Cos-7 and NIH3T3 cells were grown in DMEM containing 10% FCS and antibiotics at 37°C with 5% CO 2 . Cos-7 cells were transfected by calcium phosphate method, while the NIH3T3 cells were transfected using Lipofectin (Gibco, BRL).
One possible explanation for this result is that Ras increases the amount of p35-C/EBPβ or Elk-1 protein in the COS-7 cells, but Western blot analysis showed that both p35-C/EBPβ and Elk-1 protein levels are the same in the absence and presence of Ras (data not shown).
First, others have shown that in COS-7 cells endogenous sα i2 is not detected at PM but only found intracellularly [ 3 4 ] , although one cannot rule out that a small but functionally significant fraction of sα i2 reaches the PM.
Equal amount of total plasmids were used to transiently transfect COS-1 using the DEAE dextran method [ 40 ] . A GFP construct was used to monitor the transfection efficiency.