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Example sentences for: cos-

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Equal amount of total plasmids were used to transiently transfect COS-1 using the DEAE dextran method [ 40 ] . A GFP construct was used to monitor the transfection efficiency.
IP 3 measurements were performed on COS-1 cell cytosolic preparations using a radioreceptor assay in which IP 3 and 3H-IP 3 compete for binding to IP 3 receptors prepared from calf cerebellum membranes [ 22 23 ] .
One possible explanation for this result is that Ras increases the amount of p35-C/EBPβ or Elk-1 protein in the COS-7 cells, but Western blot analysis showed that both p35-C/EBPβ and Elk-1 protein levels are the same in the absence and presence of Ras (data not shown).
NIH 3T3 fibroblasts (from the American Type Culture Collection) and COS-7 cells (kindly provided by Dr. S. Hann, Vanderbilt University) were grown in Dulbecco's Modified Eagle Medium (DMEM) with 10% calf serum (Colorado Serum Company), 0.22% sodium bicarbonate, 4 mM L-glutamine, 25 U of penicillin G sodium per mL, and 25 mg of streptomycin per mL.
The initial experiments were performed to confirm our earlier finding that RGS3 suppressed GnRH-stimulated IP 3 production in COS-1 cells [ 9 ] and to test a truncated form of RGS3, RGS3T (RGS3 314-519) [ 24 ] for suppressive activity in the same assay (Fig.