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Example sentences for: cos-

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Construction of an open reading frame of EP 2and its expression in COS-1 cells
COS-7 cell transfections were performed by the calcium phosphate (CaPO 4 ) technique [ 31].
First, others have shown that in COS-7 cells endogenous sα i2 is not detected at PM but only found intracellularly [ 3 4 ] , although one cannot rule out that a small but functionally significant fraction of sα i2 reaches the PM.
However, the Src inhibitor, PP1 or the expression of a dominant-negative Src mutant, will reduce GPCR-mediated transphosphorylation of the EGFR and GPCR-mediated ERK activation to varying degrees (partial to total), suggesting that at least two different tyrosine kinases can be involved [ 18 ] . Src was found to co-immunoprecipitate with the phosphorylated EGFR in COS-7 cells exposed to either LPA or EGF [ 14 ] . These authors reported that the autophosphorylation of Y350 in the EGFR does not occur after LPA exposure, suggesting that c-Src is the principal tyrosine kinase in this instance and not the EGFR's intrinsic tyrosine kinase.
NIH 3T3 fibroblasts (from the American Type Culture Collection) and COS-7 cells (kindly provided by Dr. S. Hann, Vanderbilt University) were grown in Dulbecco's Modified Eagle Medium (DMEM) with 10% calf serum (Colorado Serum Company), 0.22% sodium bicarbonate, 4 mM L-glutamine, 25 U of penicillin G sodium per mL, and 25 mg of streptomycin per mL.