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Example sentences for: cos-

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RP L4/L1 transcripts, which were not significantly affected by NGF, are selectively upregulated in PC12 cells prior to 5 aza cytosine-induced death and over-expression of this gene in COS-7 cells induces apoptosis [ 39 ] . Over-expression of L7, L13A, S29 have been also reported to induce apoptosis [ 40 41 ] . In the present study, NGF promoted little if any change in expression of these RP transcripts.
Initially, we expressed the Src KM/Cas(SD) (entire Cas SD) in Cos-7 cells alone, or along with wild type (WT) v-crk, or v-crk SH2 or SH3 mutants and assayed for tyrosine phosphorylation and association by co-immunoprecipitation (Fig.
cDNA transfection of COS-1 cells and measurement of IP 3production
Cos-7 and NIH3T3 cells were grown in DMEM containing 10% FCS and antibiotics at 37°C with 5% CO 2 . Cos-7 cells were transfected by calcium phosphate method, while the NIH3T3 cells were transfected using Lipofectin (Gibco, BRL).
COS-7 cell transfections were performed by the calcium phosphate (CaPO 4 ) technique [ 31].