Words similar to cos-

Example sentences for: cos-

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cDNA transfection of COS-1 cells and measurement of IP 3production
First, others have shown that in COS-7 cells endogenous sα i2 is not detected at PM but only found intracellularly [ 3 4 ] , although one cannot rule out that a small but functionally significant fraction of sα i2 reaches the PM.
cDNA representing the rat GnRH receptor was cloned into pcDNA3 as described and validated previously [ 22 23 ] . cDNAs representing RGS3 and RGS3T (RGS3 amino acids 314-519) were cloned into the eukaryotic expression vector, rcCMV, as described [ 24 ] . COS-1 cells cultured as detailed before [ 23 ] were co-transfected with GnRH receptor cDNA and RGS3 or RGS3T cDNA using Lipofectamine (Life Technologies, Gaithersburg, MD) [ 23 ] , and 72 h later, treated with 10 -7M GnRH-A for 0, 10, 20, or 30 sec before being collected for IP 3 measurements [ 22 23 ] .
Inhibition of PGE 2 binding to the rabbit EP 2 receptor by AH13205 is about 100 times greater than that to rabbit EP 4 in transfected COS-1 cells (unpublished data).
To determine if the tyrosine phosphorylated chimera could compete with endogenous Cas for binding to endogenous c-crk, we transfected either the isolated attenuated Src kinase domain as control, or the tyrosine phosphorylated Cas substrate domain (Src*/Cas(SD)) into Cos-7 cells, and immunoprecipitated endogenous c-crk (Fig.