Words similar to cos-

Example sentences for: cos-

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Over 30 cell lines have been successfully transfected using PEI, including COS-7 cells [ 8], rat hepatocytes [ 3], human dendritic cells [ 9, 10], and mouse mammary epithelial cells [ 11].
cDNAs representing the GnRH receptor and RGS3 or RGS3T were co-transfected into COS-1 cells for GnRH-A treatment 72 h later; intracellular IP 3 concentrations were measured at 0, 10, 20, and 30 sec after addition of 10 -7M GnRH-A (Fig.
Cos-7 and NIH3T3 cells were grown in DMEM containing 10% FCS and antibiotics at 37°C with 5% CO 2 . Cos-7 cells were transfected by calcium phosphate method, while the NIH3T3 cells were transfected using Lipofectin (Gibco, BRL).
cDNA representing the rat GnRH receptor was cloned into pcDNA3 as described and validated previously [ 22 23 ] . cDNAs representing RGS3 and RGS3T (RGS3 amino acids 314-519) were cloned into the eukaryotic expression vector, rcCMV, as described [ 24 ] . COS-1 cells cultured as detailed before [ 23 ] were co-transfected with GnRH receptor cDNA and RGS3 or RGS3T cDNA using Lipofectamine (Life Technologies, Gaithersburg, MD) [ 23 ] , and 72 h later, treated with 10 -7M GnRH-A for 0, 10, 20, or 30 sec before being collected for IP 3 measurements [ 22 23 ] .
cDNA transfection of COS-1 cells and measurement of IP 3production