Words similar to cooled
Example sentences for: cooled
How can you use “cooled” in a sentence? Here are some example sentences to help you improve your vocabulary:
One microliter of the diluted product was then added to a mixture containing 12 μl deionized formamide and 0.5 μl molecular weight standard GS 350 TAMRA.The vials were denatured at 95°C/5 min, cooled on ice, and resolved using a 310 Genetic Analyzer (Perkin-Elmer).
To activate the DNA damage checkpoint, Bleomycin was added to cultures (optical density of 0.3) at a concentration of 5 mU/ml for 3 hours [ 28 ] . Thiabendazole (Sigma) was prepared in DMSO as described previously [ 45 ] and added to cooled, YE5S agar prior to pouring plates.
Metamorph acquisition software (Universal Imaging Corporation, Downingtown, PA) on an Orca cooled interline camera (Hamamatsu, Bridgewater, NJ) were used to collect the images.
The reaction was heated to 95°C for 10 seconds, and cooled to 63-68°C for 5-20 minutes, during which time OCP annealed to genomic target and was circularized by ligase.
Prior to hybridization, fragmented cRNA in a 6X SSPE-T hybridization buffer (6X: 1M NaCl, 10mM Tris pH 7.6, 0.005% Triton), was heated to 95°C for 5 min, cooled to 40°C and loaded onto an Affymetrix probe array cartridge.