Example sentences for: cooled

How can you use “cooled” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Fixation was performed by incubating samples for 10 min with cooled methanol at -20°C and washed with PBS three times.

  • Tubes were cooled on ice for 20 s between homogenizations.

  • Prior to hybridization, fragmented cRNA in a 6X SSPE-T hybridization buffer (6X: 1M NaCl, 10mM Tris pH 7.6, 0.005% Triton), was heated to 95°C for 5 min, cooled to 40°C and loaded onto an Affymetrix probe array cartridge.

  • The reaction was cooled to 4°C, and 20 μl ERCA reaction mix was added (typically 16 units BST polymerase (New England Biolabs, Beverly, MA), 6 mM dNTPs, 0.5 μM P1, 0.5 μM P2, 4 μM Q-PNA, 7.5 μM TMAO in 1x ThermoPol Buffer II, all concentrations final).

  • USAT says that although Bill Gates has held talks at his house regarding this matter with AT&T execs, the phone company has since cooled on the deal because "it may not want to be an owner of Microsoft's money-losing Web sites."


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