Example sentences for: cooled

How can you use “cooled” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To a solution of 6 (50 mg, 87 μmol) in dichloromethane (2 mL) cooled to 0°C was added m- chloroperoxybenzoic acid (CPBA, 26 mg, 93 μmol) in 0.5 mL of dichloromethane.

  • This tissue was mounted in TBS tissue freezing medium (Triangle Biomedical Sciences, Durham, NC), frozen in isopentane cooled in liquid nitrogen, and stored at -80°C.

  • At the typical unit, hot corrosive flue gases leaving the particulate control device (149- 182 ºC) are cooled, or quenched, before entering the main absorber device.

  • The RNA/primer mixture was denatured at 70°C for 3 min and cooled on ice for 2 min, followed by adding: 4 μl of 5X first strand buffer (Invitrogen™), 2 μl 0.1 M DTT, 1 μl RNasin ®(40 U/μl, Promega™), 2 μl 10 mM dNTP, and 2 μl Superscript™ II (200 U/μl, Invitrogen™), and incubated at 42°C for 1.5 hours.

  • Human muscle biopsy samples were diagnostic specimens flash-frozen immediately after surgery in isopentane cooled in liquid nitrogen, with storage in small, airtight, humidified tubes at -80°C until RNA isolation.


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