Example sentences for: cooled

How can you use “cooled” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • One microliter of the diluted product was then added to a mixture containing 12 μl deionized formamide and 0.5 μl molecular weight standard GS 350 TAMRA.The vials were denatured at 95°C/5 min, cooled on ice, and resolved using a 310 Genetic Analyzer (Perkin-Elmer).

  • To activate the DNA damage checkpoint, Bleomycin was added to cultures (optical density of 0.3) at a concentration of 5 mU/ml for 3 hours [ 28 ] . Thiabendazole (Sigma) was prepared in DMSO as described previously [ 45 ] and added to cooled, YE5S agar prior to pouring plates.

  • Metamorph acquisition software (Universal Imaging Corporation, Downingtown, PA) on an Orca cooled interline camera (Hamamatsu, Bridgewater, NJ) were used to collect the images.

  • The reaction was heated to 95°C for 10 seconds, and cooled to 63-68°C for 5-20 minutes, during which time OCP annealed to genomic target and was circularized by ligase.

  • Prior to hybridization, fragmented cRNA in a 6X SSPE-T hybridization buffer (6X: 1M NaCl, 10mM Tris pH 7.6, 0.005% Triton), was heated to 95°C for 5 min, cooled to 40°C and loaded onto an Affymetrix probe array cartridge.


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