Words similar to control-infected
Example sentences for: control-infected
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In contrast, cell-cell contact in wild-type and control-infected, but not HIV-1-infected podocytes, caused a severe down-regulation of cyclin D 1 transcript and protein concomitant with the up-regulation of synaptopodin, a podocyte differentiation marker [ 7 28 ] , by the end of the 14-day differentiation period.
Protein (250 μg) from the control-infected or HIV-1-infected podocytes on day 14 of the differentiation protocol was also analyzed by western blot for total pRb (ECL detection of a 1:1000 dilution of rabbit anti-Rb, Santa Cruz Biotechnology) and phospho-Rb (Ser780) (ECL detection of a 1:500 dilution of rabbit anti-phospho-Rb (Ser780) antibody binding, Cell Signaling).
Moreover, HIV-1-infected podocytes, but not control-infected podocytes, had readily detectable phospho-pRb (Ser780) at the end of the differentiation period, suggesting that the stably up-regulated cyclin D 1 protein remained active in promoting cell-cycle progression (Figure 2B).
Total RNA and protein were extracted from wild-type, control-infected, and HIV-1 infected podocytes during the Reiser, et al.