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Another application of this method to a drusen image is seen in Fig 4. The first frame is the standardized color image, which then was contrast-enhanced in Photoshop for ease of drusen visualization (middle frame).
Each such drusen image is superimposed on the contrast-enhanced image.
After background leveling, the optimum threshold level for drusen segmentation in the selected subfield is chosen by flicker comparison with the contrast-enhanced image, as follows.
All further analysis and drusen segmentation were hence carried out on the green channel of the standardized image, with the full color contrast-enhanced versions used for subjective comparison.
The optimized threshold is selected by visually inspecting the correspondence of the boundaries of the segmented drusen objects to those of the contrast-enhanced objects.