Words similar to contig
Example sentences for: contig
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The following files are available with the online version of this article: the e-values of the best matches for the initial BLASTX searches against bacteria, C. elegans and Drosophila for the WMi dataset (Additional data file 1), together with a mapping file (Additional data file 2) that gives the MI contig number associated with each filename; the best match and e-value for the BLASTX search of the WMj dataset against bacteria (Additional data file 3), C. elegans (Additional data file 4) and Drosophila (Additional data file 5); the e-value for the best match to bacteria, C. elegans and Drosophila resulting from a TBLASTX search for the NMi dataset (Additional data file 6), the NMj dataset (Additional data file 7), the NMh dataset (Additional data file 8), where a value of 100 indicates no match found; a text file with details of the data given in each of these dataset files (Additional data file 9); the alignment in Phylip format used to calculate the NodL tree (Additional data file 10); the alignment in Phylip format of the original sequences, before any manual adjustments were made (Additional data file 11); and a key giving the gi number listed in the alignment for each species (Additional data file 12).
BAC contig construction
Misassemblies were recognized by: regions of high quality unaligned sequence; multiple runs of poly(A) and/or poly(T) (at least 15 nucleotides with no more than a one non-A/T base); internal poly(A) and/or poly(T) runs (> 50 nucleotides from either end of a contig and ≥ 15 or more nucleotides long with no more than one non-A/T base; internal stretches of low consensus quality (> 30 nucleotides from either end of a contig and ≥ 50 nucleotides where 90% of the nucleotides had a consensus quality below Phred 20).
We confirmed the sequence quality of the genomic region encompassing CG018314 (12,731 bp) by independently assembling an 18,284 bp contig consisting solely of whole-genome shotgun (WGS) traces.
The most extensively studied HGT candidates are four genes encoding β-1,4-endoglucanase, initially identified in the cyst nematodes G. rostochiensis and H. glycines [ 18 19 ] . These four genes (NemaGene Contig IDs MI00537, MI01011, MI01381 and MI01842) [ 30 ] appear to define two sets of paralogs formed before divergence of the cyst and root-knot nematodes.
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