Words similar to constructs
Example sentences for: constructs
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Both regions contained multiple open reading frames; however, we focused immediately on a single gene for each mutant, SEC13 for npa2-1 and SEC23 for npa1-1 . SEC13 was first identified in a screen for secretory mutants and encodes a protein of the COPII coat, which is required for vesicle budding from the ER [ 37 38 39 ] . More recently, it has been shown that a fraction of the total cellular Sec13p co-fractionates with NPCs in a distinct subcomplex with Nup84p, Nup85p, Nup120p, Nup145Cp and Seh1p [ 40 41 ] . SEC23 was also identified in a screen for secretory mutants, and it, too, is a component of the COPII coat [ 37 38 39 ] . Co-purification of Sec23p with intact NPCs has not been reported [ 5 ] ; however, Sec23p was recently isolated in Nup42p and Nup116p affinity chromatography strategies [ 42 ] . Expression constructs containing only SEC13 or SEC23 were transformed into the mutant strains and assayed for their ability to rescue growth at the non-permissive temperature.
GST fusions encoding the Rlk amino-terminal and SH3 domains or the amino-terminus alone (containing the cysteine string and proline-rich region) bound biotinylated GST fusions of the SH3 domains of Fyn, Hck, and Lyn, but not those of Src or Lck, despite the ability of these constructs to bind other proline rich sequences (Fig.
[ 34 ] found AA to be more potent than DHA (saturated and monounsaturated being ineffective) at inhibiting activation of SREBP-1c promoter and LXRE enhancer constructs, induced by co-expression of LXRα or -β.
The vector for all constructs was pBluescript(-) (Stratagene).
All Drosophila expression constructs were cloned in the vector pPacPL [ 27 28 ] . Cells grown in 6-well plates were transfected with 0.5 μg of indicated plasmid together with the reporter plasmids using Cellfectin reagent (Invitrogen Life Technologies).
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