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Example sentences for: constructs
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High levels of OPN in breast tumor cell lines appear to correlate with a more aggressive in vivo phenotype [ 2 20 ] . Transfection of OPN expression constructs into cells producing noninvasive mammary epithelial tumors has been reported to convert them to a malignant phenotype [ 8 21 ] . Furthermore, transfection of antisense OPN constructs or OPN-targeting ribozymes into transformed tumorigenic cells can result in impaired anchorage-independent growth, as well as in diminished tumor-forming ability in vivo [ 22 23 ] . OPN is also typically overexpressed in human tumors, including breast cancer [ 15 24 ] . However, the source of OPN in primary breast tumors was originally thought to be infiltrating macrophages rather than tumor cells themselves [ 15 ] .
These constructs were injected into D. melanogaster y;w embryos by standard P -element transformation [ 82].
Full-length Rlk cDNA expression constructs pCIR, pcDNA3Rlk and Rlk-GFP have been described previously [ 19].
All of the constructs that have significant levels of promoter activity contain the major transcriptional start sites identified in this study [ 33 34 35 36 ] . Mouse p27 Kip1reporter constructs containing ~500 base pairs upstream of the ATG start codon had virtually no activity [ 33 34 ] . Similarly a human p27 Kip1reporter construct containing 435 base pairs upstream of the ATG was inactive [ 35 ] . Our data indicate that this is because these constructs did not carry the major transcription initiation sites or the promoter sequences necessary to position the transcription complex.
GST fusions encoding the Rlk amino-terminal and SH3 domains or the amino-terminus alone (containing the cysteine string and proline-rich region) bound biotinylated GST fusions of the SH3 domains of Fyn, Hck, and Lyn, but not those of Src or Lck, despite the ability of these constructs to bind other proline rich sequences (Fig.
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