Words similar to constructs
Example sentences for: constructs
How can you use “constructs” in a sentence? Here are some example sentences to help you improve your vocabulary:
96 colonies were picked and 27 screened, for electroporations with each of the two constructs R26R-YFP and R26R-CFP.
Transfections with constructs containing successive deletions of the 3 kb fragment show that removal of DNA regions upstream of a StuI site (at -215, relative to the CD5 protein initiation methionine codon) does not significantly decrease luciferase expression from the level obtained with the full length fragment.
Subcloning of NRG1 constructs
[ 34 ] found AA to be more potent than DHA (saturated and monounsaturated being ineffective) at inhibiting activation of SREBP-1c promoter and LXRE enhancer constructs, induced by co-expression of LXRα or -β.
Both regions contained multiple open reading frames; however, we focused immediately on a single gene for each mutant, SEC13 for npa2-1 and SEC23 for npa1-1 . SEC13 was first identified in a screen for secretory mutants and encodes a protein of the COPII coat, which is required for vesicle budding from the ER [ 37 38 39 ] . More recently, it has been shown that a fraction of the total cellular Sec13p co-fractionates with NPCs in a distinct subcomplex with Nup84p, Nup85p, Nup120p, Nup145Cp and Seh1p [ 40 41 ] . SEC23 was also identified in a screen for secretory mutants, and it, too, is a component of the COPII coat [ 37 38 39 ] . Co-purification of Sec23p with intact NPCs has not been reported [ 5 ] ; however, Sec23p was recently isolated in Nup42p and Nup116p affinity chromatography strategies [ 42 ] . Expression constructs containing only SEC13 or SEC23 were transformed into the mutant strains and assayed for their ability to rescue growth at the non-permissive temperature.
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