Words similar to consed
Example sentences for: consed
How can you use “consed” in a sentence? Here are some example sentences to help you improve your vocabulary:
Contigs flagged for possible misassembly were manually edited in Consed and potentially chimeric ESTs and other suspect ESTs were identified and removed from the pool of traces.
Although Phrap is a program intended for genome assembly, it has been applied previously to ESTs with modifications [ 90 ] . To determine initial assembly quality, the largest contigs were inspected using the assembly viewer Consed [ 91 ] . Misassemblies bringing unrelated ESTs together into giant contigs usually resulted from the alignment of long poly(A) tails.
Autofinish (part of the consed computer software package) was used to automatically design custom primers [ 10] for clones that needed quality improvement.
With an estimated gap size, LBNL used one of five gap-filling methods to determine their sequence: first, gaps less than 500 bp were sequenced using custom primers designed by consed and a 3-kb or 10-kb plasmid as DNA template; second, gaps between 500 bp and 2.5 kb that were spanned by a 3-kb clone were completed by sequencing the 3-kb clone using a transposon-based sequencing strategy, described below; third, gaps between 2.5 kb and 10 kb that were spanned by a 10-kb clone were completed by sequencing the 10-kb clone using a transposon-mediated sequencing strategy; fourth, gaps larger than 10 kb were completed by sequentially sequencing multiple 10-kb clones to walk across the gap.
All contigs with more than three member ESTs was screened for misassemblies using Consed tools and newly written scripts.
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