Words similar to conceptuses
Example sentences for: conceptuses
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Following completion of conceptus elongation, IL-1β gene expression and secretion rapidly decline and expression levels are more than 2000-fold lower in day 15 compared to day 12 filamentous conceptuses.
Numerous transcripts were up-regulated in conceptuses of filamentous morphology including, pro-interleukin-1β, interleukin-1β (IL-1β), s-adenosylhomocysteine hydrolase (SAHH), heat shock cognate 70 kDa protein (HSC70), metallopanstimulin-1 (MPS-1) and elfin.
An autocrine effect of conceptus estrogen synthesis has been suggested as filamentous conceptuses have greater gene expression for estrogen receptor β compared to its spherical and tubular counterparts [ 9 ] .
SAHH may have significant impact on the conceptuses ability to use folates during this transitional stage of development.
Total RNA was extracted from individual conceptuses and pools of conceptuses of identical morphologies as previously described in our laboratory [ 8 ] . Briefly, conceptuses were denatured for 15 min on ice using 500 μl of denaturing solution (4 M guanidinium thiocyanate, 25 mM sodium citrate, pH 7.0, 0.5% sarcosyl, 0.1 M 2-β-mercaptoethanol), 500 μl phenol, 70 μl 2 M sodium acetate (pH 4.0), and 140 μl chloroform/iso-amyl-alcohol (49:1 fresh dilution).
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