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1%) indicated the national origins of the sample with only one study comprising subjects from the United States (1.
The RNA was denatured prior to loading by incubating the RNA at 65°C for 10 min in a loading buffer comprising 1X MOPS, 50% formamide, 6.5% formaldehyde, 5% glycerol, 0.1 mM EDTA, 0.025% bromophenol blue, 0.025% xylene cyanol.
--comprising guys who have gained 50 pounds since high school, guys who know their way around a search engine but, what with the wife and the child support and the gas bills and all those $7 bottles of wine, lack the loose dollars and idle time that can buy true American style.
The pattern classification system used in this study had been derived from a scheme originally developed to classify timeline rhythms (bell patterns) in the music of African origin [ 43 ] . In a previous publication we constructed a scheme consisting of 42 pattern classes comprising all binary patterns with 3 to 8 bits (pulses, heartbeats) [ 19 20 ] . Later, the scheme was extended to 47 pattern classes to also include the most relevant pattern classes with up to 12 bits (applied in [ 21 22 ] ). Many of these binary patterns are associated with RSA and are generated constantly and repeatedly when phase coordination between heart beat and respiration is present (see Table 3).
8. Traditionally, retirement income was characterized as a "three-legged stool" comprising Social Security, employer pensions, and individuals' own saving for retirement.