Words similar to coculture
Example sentences for: coculture
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With respect to osteoclast formation, IFN-γ is known to potently inhibit RANKL-mediated osteoclastogenesis in both spleen-derived macrophage cultures and bone marrow coculture systems [ 19 20 21 ] . It has also been demonstrated that mice defective in IFN-γ signaling have a more rapid onset of arthritis and bone resorption compared with wild-type mice, suggesting a protective role of IFN-γ in early arthritis [ 21 22 ] .
Direct coculture of hESMPCs with C2C12 cells led to the formation of hESMPC-derived myotubes, as visualized by expression of human nuclear antigen (Figure 3B), similar to those formed by host C2C12 cells.
Human cell contribution to myotubes in coculture was confirmed by expression of human muscle-specific transcripts such as MyoD, myosin heavy chain IIa , and myogenin (data not shown).
After 1 wk of coculture, myotubes composed of human nuclei accounted for more than 10% of the total number of human cells present, and each human myotube was composed of up to ten human nuclei.
After 40 d of coculture, cells were harvested and sorted by FACS for CD73, a surface marker expressed in adult MSCs [5] (Figure 1A).