Words similar to co-immunoprecipitate
Example sentences for: co-immunoprecipitate
How can you use “co-immunoprecipitate” in a sentence? Here are some example sentences to help you improve your vocabulary:
Similarly, GPI-linked proteins such as CD 109, TM4SF proteins such as CD81, and other transmembrane proteins such as CD147/EMMPRIN can be found at substantial levels in light membrane fractions [ 44 45 ] , and our unpublished results), and these also did not co-immunoprecipitate with CD98 (Fig.
Src has also been found to co-immunoprecipitate with the agonist-stimulated β 2 -AR during the period of ERK activation [ 8 ] . Our previous work [ 11 13 ] suggested that a Src-like protein tyrosine kinase was primarily responsible for tyrosine phosphorylation of the δ-OR and ERK activation induced by DTLET when the receptor was expressed in CHO or HEK-293 cells, because both responses were blocked by the presence of 50 μM PP1.
However, the Src inhibitor, PP1 or the expression of a dominant-negative Src mutant, will reduce GPCR-mediated transphosphorylation of the EGFR and GPCR-mediated ERK activation to varying degrees (partial to total), suggesting that at least two different tyrosine kinases can be involved [ 18 ] . Src was found to co-immunoprecipitate with the phosphorylated EGFR in COS-7 cells exposed to either LPA or EGF [ 14 ] . These authors reported that the autophosphorylation of Y350 in the EGFR does not occur after LPA exposure, suggesting that c-Src is the principal tyrosine kinase in this instance and not the EGFR's intrinsic tyrosine kinase.
Studies have shown that the sitosterolemic patients hyperabsorb all sterols (cholesterol and phytosterols) and fail to excrete phytosterols into bile [ 3 4 5 6 7 ] . Positional mapping of the STSL locus to human chromosome 2p21 led to the identification of two genes, ATP binding cassette (ABC) half-transporters G5 and G8 that were mutated in affected individuals [ 8 9 10 11 12 ] . The protein products of these two genes (sterolin-1 and sterolin-2 respectively) are thought to form heterodimers to constitute a functional transporter, based primarily on the genetic evidence that affected individuals are either completely mutated in sterolin-1 or sterolin-2, but not both [ 12 ] . More recently, evidence from in vitro expression of these proteins has been presented that these two proteins co-immunoprecipitate, supporting this conclusion [ 13 ] . ABCG5 and ABCG8 appear to be present in all mammalian genomes examined to date and are highly conserved between species, indicating that these genes may have a similar function in other species.
It is also of note that we can reproducibly co-immunoprecipitate Rlk and Fyn, perhaps due to their association with RAFTs, whereas we have been unable to co-precipitate Itk with Src family kinases when co-expressed in 293T cells (data not shown), again demonstrating distinct properties of the Rlk protein compared to certain other members of the Btk family.
Loading...