Words similar to cmv-lacz

Example sentences for: cmv-lacz

How can you use “cmv-lacz” in a sentence? Here are some example sentences to help you improve your vocabulary:

Biotinylated Ad5.CMV-LacZ, prepared by treatment with 15 μg/ml sulfo-NHS-LC-biotin as above, was diluted in PBST and incubated for immobilization in streptavidin-coated wells (well diameter, 0.64 cm; Reacti-Bind Streptavidin Coated Polystyrene Wells, Pierce Chemical) (50 μl per well) for 2 h at 25°C with shaking on a rotary shaker at 150 rpm.
The recombinant adenoviral vectors used are Ad5.CMV-LacZ (Qbiogene), which is derived from adenovirus serotype 5 with the deletion of the viral E1A, E1B, and E3 genes.
The maximum infectivity was seen with Ad5.CMV-LacZ that had been treated with 15 μg/ml sulfo-NHS-LC-biotin, where the infectivity was approximately 75% of that of unmodified Ad5.CMV-LacZ used free in solution.
For control experiments, a solution containing free, unmodified Ad5.CMV-LacZ (50 μl per well) was applied to target cells grown on streptavidin-coated wells.
Up to this number of viral particles per well, used for immobilization for biotinylated Ad5.CMV-LacZ or for transduction by free Ad5.CMV-LacZ in solution, immobilized adenoviral vectors showed lower infectivity than free viral vectors.