Example sentences for: cmv-lacz

How can you use “cmv-lacz” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After the removal of unbound Neutralite avidin, Ad5.CMV-LacZ, which had been biotinylated by treatment with 15 μg/ml sulfo-NHS-LC-biotin as above, was applied to the wells and allowed to bind to unoccupied biotin-binding sites of Neutralite avidin that had been bound to biotin moieties on the solid surface.

  • Up to this number of viral particles per well, used for immobilization for biotinylated Ad5.CMV-LacZ or for transduction by free Ad5.CMV-LacZ in solution, immobilized adenoviral vectors showed lower infectivity than free viral vectors.

  • A relatively constant number of viral particles was bound to the well surface at each addition cycle of biotinylated Ad5.CMV-LacZ.

  • For the infectivity analysis of biotinylated Ad5.CMV-LacZ, dilutions of biotinylated viral vectors were placed over monolayers of D-17 target cells on a 96-well cell culture plate (8 × 10 3cells per well), followed by the incubation of cells at 37°C for 48 h.

  • In contrast, when C6 cells were cultured directly on streptavidin-coated wells, on which Ad5.CMV-LacZ had been immobilized, considerable amounts of infection were seen when 2.5 × 10 6viral particles per well and greater were added to wells at the immobilization step.


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