Words similar to cmv-lacz
Example sentences for: cmv-lacz
How can you use “cmv-lacz” in a sentence? Here are some example sentences to help you improve your vocabulary:
After the removal of unbound Neutralite avidin, Ad5.CMV-LacZ, which had been biotinylated by treatment with 15 μg/ml sulfo-NHS-LC-biotin as above, was applied to the wells and allowed to bind to unoccupied biotin-binding sites of Neutralite avidin that had been bound to biotin moieties on the solid surface.
Up to this number of viral particles per well, used for immobilization for biotinylated Ad5.CMV-LacZ or for transduction by free Ad5.CMV-LacZ in solution, immobilized adenoviral vectors showed lower infectivity than free viral vectors.
A relatively constant number of viral particles was bound to the well surface at each addition cycle of biotinylated Ad5.CMV-LacZ.
For the infectivity analysis of biotinylated Ad5.CMV-LacZ, dilutions of biotinylated viral vectors were placed over monolayers of D-17 target cells on a 96-well cell culture plate (8 × 10 3cells per well), followed by the incubation of cells at 37°C for 48 h.
In contrast, when C6 cells were cultured directly on streptavidin-coated wells, on which Ad5.CMV-LacZ had been immobilized, considerable amounts of infection were seen when 2.5 × 10 6viral particles per well and greater were added to wells at the immobilization step.