Words similar to cmv-lacz
Example sentences for: cmv-lacz
How can you use “cmv-lacz” in a sentence? Here are some example sentences to help you improve your vocabulary:
In contrast, substantial numbers of D-17 cells were transduced when Ad5.CMV-LacZ was treated with 3 - 30 μg/ml sulfo-NHS-LC-biotin.
Varying numbers of biotinylated Ad5.CMV-LacZ, prepared by treatment with 15 μg/ml sulfo-NHS-LC-biotin, were applied to streptavidin-coated wells (well diameter, 0.64 cm) for immobilization, followed by the removal of unbound viral particles.
Biotinylated Ad5.CMV-LacZ, prepared by treatment with 15 μg/ml sulfo-NHS-LC-biotin as above, was diluted in PBST and incubated for immobilization in streptavidin-coated wells (well diameter, 0.64 cm; Reacti-Bind Streptavidin Coated Polystyrene Wells, Pierce Chemical) (50 μl per well) for 2 h at 25°C with shaking on a rotary shaker at 150 rpm.
After the removal of unbound Neutralite avidin, Ad5.CMV-LacZ, which had been biotinylated by treatment with 15 μg/ml sulfo-NHS-LC-biotin as above, was applied to the wells and allowed to bind to unoccupied biotin-binding sites of Neutralite avidin that had been bound to biotin moieties on the solid surface.
The recombinant adenoviral vectors used are Ad5.CMV-LacZ (Qbiogene), which is derived from adenovirus serotype 5 with the deletion of the viral E1A, E1B, and E3 genes.
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