Words similar to cmv-lacz
Example sentences for: cmv-lacz
How can you use “cmv-lacz” in a sentence? Here are some example sentences to help you improve your vocabulary:
Biotinylated Ad5.CMV-LacZ, prepared by treatment with 15 μg/ml sulfo-NHS-LC-biotin as above, was diluted in PBST and added to streptavidin-coated wells (2.
This result is in contrast with our previous study, which showed that greater than 95% of biotinylated Ad5.CMV-LacZ, prepared under the same conditions, can be readily captured on streptavidin-coated microbeads [ 10 ] . Thus, the relatively low immobilization efficiency of biotinylated Ad5.CMV-LacZ on the surface of streptavidin-coated wells is not attributable to the absence of accessible biotin moieties on the surfaces of a fraction of viral particles or the presence of unreacted biotinylation reagent in the biotinylated Ad5.CMV-LacZ preparations.
For control experiments, a solution containing free, unmodified Ad5.CMV-LacZ (50 μl per well) was applied to target cells grown on streptavidin-coated wells.
Biotinylated Ad5.CMV-LacZ (1 × 10 7viral particles per spot), prepared by treatment with 15 μg/ml sulfo-NHS-LC-biotin as above, were incubated in the spot areas within plastic rings at 25°C for 2 h, followed by washing the wells with PBST to remove unbound viral particles.
2above, only about 15 - 40% of biotinylated Ad5.CMV-LacZ applied can actually be immobilized on streptavidin-coated wells.