Words similar to cmv-lacz

Example sentences for: cmv-lacz

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Known numbers of biotinylated Ad5.CMV-LacZ particles were incubated in streptavidin-coated wells for immobilization, and the number of viral particles remaining unbound in the solution was estimated from their infectivity on D-17 cells (Fig.
After the removal of unbound Neutralite avidin, Ad5.CMV-LacZ, which had been biotinylated by treatment with 15 μg/ml sulfo-NHS-LC-biotin as above, was applied to the wells and allowed to bind to unoccupied biotin-binding sites of Neutralite avidin that had been bound to biotin moieties on the solid surface.
4A) for both immobilized and free adenoviral vectors, although free Ad5.CMV-LacZ showed higher infectivity than immobilized adenoviral vectors.
Ad5.CMV-LacZ was treated with varying concentrations (0 - 30 μg/ml) of sulfo-NHS-LC-biotin, and non-virion-associated biotinylation reagent was removed by repeated ultrafiltration.
To test this, a constant number of fresh biotinylated Ad5.CMV-LacZ was applied to the same streptavidin-coated well repeatedly (2.