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Example sentences for: clendo

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For Western blot analysis, CLENDO cells were treated with TNFα (50 ng/ml) or C2 ceramide for 0, 2, 5, 10, 30, or 60 min.
. Treatment of CLENDO cells with an exogenous source of ceramide (C2, 50 μM) caused a significant decrease in cell viability starting 24 h after treatment compared to untreated cells cultured for 72 h (Fig.
To identify apoptosis-inducing agents in CLENDO cells, culture medium was removed following treatments, cells were washed one time with ice cold PBS, and fixed for 10 min with 4% paraformaldehyde.
Treatment with GSH alone had no effect on the CLENDO cell viability.
Interestingly it has been demonstrated that rapid production of ROS in endothelial cells exposed to TNFα is mediated by ceramide [ 32 ] . Ceramide is a central figure in the sphingomyelin pathway, which has also been linked to stress related signaling and apoptosis [ 33 34 ] . This report provides evidence that cytokines regulate the fate of CLENDO cells.