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Example sentences for: clendo

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Furthermore, to determine if S1P attenuated ceramide- or TNFα-induced cell death, CLENDO cells were first pretreated with S1P for 30 min, followed by application of ceramide (50 μM) or TNFα (50 ng/ml).
In a second group of experiments, we observed that CLENDO cells were resistant to apoptosis when treated for 72 h with sFasL or the human Fas activating antibody CH-11.
The amount of ceramide generated by CLENDO cells in response to TNFα treatment (50 ng/ml) was determined in three independent sets of experiments via the diacyl glycerol kinase assay as described [ 26 40 ] . Each experiment contained an untreated control that was used for comparison with TNFα treated samples.
. Treatment of CLENDO cells with an exogenous source of ceramide (C2, 50 μM) caused a significant decrease in cell viability starting 24 h after treatment compared to untreated cells cultured for 72 h (Fig.
To determine which cytokines initiate apoptosis in CLENDO cells we treated the cells for 48 h with TNFα, IFNγ, or the combination of the two.