Words similar to clb
Example sentences for: clb
How can you use “clb” in a sentence? Here are some example sentences to help you improve your vocabulary:
dbf2Δ [ 22 7 ] . To address if production of new proteins is essential for exit from mitosis, we examined the effects of the protein synthesis inhibitor cycloheximide on Clb2 degradation, a key aspect of this process.
In the absence of cycloheximide, Sic1 accumulated and Clb2 disappeared as cells exited mitosis (Figure 1, lanes 1-4).
tem1Δ by enabling Tem1-independent degradation of Clb2 and accumulation of Sic1.
We first tested Cdc5, because Cdc5 is required for Cdc14 release (Figure 1), and because overexpression of Cdc5 triggers ectopic degradation of Clb2 [ 19 20 ] , which normally depends upon active Cdc14 [ 3 ] . These experiments employed a GAL-CDC5(DB▲) strain, in which expression of Cdc5(DB▲), a stabilized version of Cdc5 lacking the destruction box, was driven by the inducible GAL promoter [ 20 ] . Cells were arrested in G1 by α-factor, supplemented with galactose to induce the GAL promoter, and released into galactose-containing medium.
Having established that tab3-1 indeed bypassed Tem1 deficiency at a cellular level, we tested whether Sic1 accumulation and Clb2 degradation, two hallmark events that accompany exit from mitosis, also occurred.
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