Words similar to clb

Example sentences for: clb

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Detection of proteins from crude yeast extracts was as described [ 15 ] . The following primary antibodies were used in immunoblotting: anti-Sic1 (1:8000), anti-Clb2 (1:2500), anti-Cdc28 (1:3000), and 12CA5 (directed against the haemagglutinin (HA) epitope; 1:1000, with 0.5 M NaCl).
Sic1) normally facilitates the exit from mitosis, it reveals that a key aspect of mitotic exit - inactivation of Cdc28 protein kinase via degradation of the mitotic cyclin Clb2 - can proceed beyond the Cdc15-dependent step without synthesis of new proteins.
Importantly, tab mutants from both classes appeared to bypass mitotic arrest in Tem1-depleted cells by enabling degradation of Clb2 and accumulation of Sic1 - two hall-marks of mitotic exit (Figure 3; [ 15 18 ] ).
Having established that tab3-1 indeed bypassed Tem1 deficiency at a cellular level, we tested whether Sic1 accumulation and Clb2 degradation, two hallmark events that accompany exit from mitosis, also occurred.
tem1Δ by enabling Tem1-independent degradation of Clb2 and accumulation of Sic1.