Words similar to cin
Example sentences for: cin
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High-molecular weight DNA from frozen tumor and the corresponding peripheral blood specimens, and frozen cells from cell lines was isolated as previously described [ 25 ] . Tumor cells from paraffin-embedded CIN tissue specimens were isolated by laser capture microdissection (Arcturus, Mountain View, CA) after methyl green-staining and the extraction of DNA as described earlier [ 26 ] . Total RNA was isolated from semi-confluent cell cultures using the TRIzol reagent (Life Technologies, Gaithersburg, MD) according to the manufacturer's protocol.
5p deletions also have been reported in precursor CIN lesions with the frequencies between 14.
[ 13 ] . HPV was not detected by the assay in basal/parabasal cells of (non-condylomatous) CIN I lesions (> 30 examined; unless HPV-16 positive [ 2 ] ) suggesting low-copy number episomal HPV was not detectable and indicating a limit to the sensitivity of the assay.
Twenty-five CIN specimens were classified as high-grade and one as low-grade lesions.
Converging evidence from epidemiological and molecular studies suggests that infection of genital human papillomavirus (HPV) is causally linked to the development of CC [ 4 ] . Since only a small fraction of HPV-infected cervical intra-epithelial neoplastic (CIN) lesions progress to invasive cancer, these studies further suggest that in addition to HPV, other host genetic factors play a role in cervical carcinogenesis [ 5 ] . A number of molecular studies have identified genetic alterations in these two histologic types of CC and at various stages of precursor lesions [ 6 7 8 ] . Despite this molecular characterization, the genetic basis of CC initiation and progression is still very poorly understood.
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