Words similar to cht
Example sentences for: cht
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Appropriate pairs of gene and element primers were used to detect the presence or absence of the respective retrotransposon inserts associated with each gene in strains representing 12 geographically dispersed populations of D. melanogaster . The results presented in Figure 2and Table 1demonstrate that while the Burdock insertion located just 3' to cathD gene is not present in any of 12 strains representing a geographically diverse sampling of natural populations, the Antonia LTR fragment located in the intron of the heterochromatic Cht3 gene is fixed in all 12 strains tested.
The two Cht3 intron fragments descibed in Figure 3 have the following provisional accession numbers in GenBank:
9%, Figure 4), is higher than that for the LTR containing Cht3 intron (1.
The PCR products for primer set cht3(f2) and LTR(r) were amplified in a 25 μl reaction containing Expand Long Template PCR System 10X PCR buffer #1 supplied by Roche (Indianapolis, IN), 0.35 mM dNTPs, 0.32 μM of each primer, and 1.3 U of Expand Long Template PCR System DNA polymerase mix supplied by Roche (Indianapolis, IN).
The PCR products for primer set cht3(f) and cht3(r) and primer set Antonia LTR(f) and Antonia LTR(r) were amplified in a 25 μl reaction containing 3 mM MgCl 2 , 10X PCR buffer supplied by Pierce (Rockford, IL), 5% DMSO, 0.2 mM dNTPs, 0.5 μM of each primer, and 0.5 U of Taq DNA polymerase supplied by Pierce [Rockford, IL].