Words similar to cht
Example sentences for: cht
How can you use “cht” in a sentence? Here are some example sentences to help you improve your vocabulary:
The reaction mix and program used for all sets of primers are the same as those described for primer set cht3(f) and cht3(r) and primer set Antonia LTR(f) and Antonia LTR(r) in the Cht3 PCR (above).
The program consisted of an initial incubation at 94°C for 3 min for 1 cycle, a 30 cycle extension at 94°C for 30 sec, 56°C for cht3(f)/cht3(r) primer set or 57°C for Antonia LTR(f)/Antonia LTR(r) primer set for 30 sec, 72°C for 1 min 30 sec, and a 1 cycle final extension of 72°C for 5 min.
The PCR products for primer set cht3(f) and cht3(r) and primer set Antonia LTR(f) and Antonia LTR(r) were amplified in a 25 μl reaction containing 3 mM MgCl 2 , 10X PCR buffer supplied by Pierce (Rockford, IL), 5% DMSO, 0.2 mM dNTPs, 0.5 μM of each primer, and 0.5 U of Taq DNA polymerase supplied by Pierce [Rockford, IL].
The results presented here are consistent with the hypothesis that a 359 bp fragment of the Antonia retrotransposon located within the intron of the heterochromatic Drosophila melanogaster Cht3 gene may be of adaptive evolutionary significance.
Appropriate pairs of gene and element primers were used to detect the presence or absence of the respective retrotransposon inserts associated with each gene in strains representing 12 geographically dispersed populations of D. melanogaster . The results presented in Figure 2and Table 1demonstrate that while the Burdock insertion located just 3' to cathD gene is not present in any of 12 strains representing a geographically diverse sampling of natural populations, the Antonia LTR fragment located in the intron of the heterochromatic Cht3 gene is fixed in all 12 strains tested.
Loading...