Example sentences for: cht

How can you use “cht” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The program consisted of an initial incubation at 94°C for 3 min for 1 cycle, a 30 cycle extension at 94°C for 30 sec, 56°C for cht3(f)/cht3(r) primer set or 57°C for Antonia LTR(f)/Antonia LTR(r) primer set for 30 sec, 72°C for 1 min 30 sec, and a 1 cycle final extension of 72°C for 5 min.

  • The PCR products for primer set cht3(f2) and LTR(r) were amplified in a 25 μl reaction containing Expand Long Template PCR System 10X PCR buffer #1 supplied by Roche (Indianapolis, IN), 0.35 mM dNTPs, 0.32 μM of each primer, and 1.3 U of Expand Long Template PCR System DNA polymerase mix supplied by Roche (Indianapolis, IN).

  • Cht3 PCR

  • The reaction mix and program used for all sets of primers are the same as those described for primer set cht3(f) and cht3(r) and primer set Antonia LTR(f) and Antonia LTR(r) in the Cht3 PCR (above).

  • A total of 685 bp of the Cht3 intron was sequenced.


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