Words similar to chondroitinase

Example sentences for: chondroitinase

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The soluble material was separated by centrifugation (3000 × g , 10 min) and assayed for absorbance at 235 nm to measure the concentration of product Δ 4-hexuronate (Molar extinction coefficient = 5500; [ 33 ] ). For the chondroitinase ABC reactions, each chondroitin sulfates A, B and C substrate (1 mg each) and concentrated CM, were incubated with 0.12 units of chondroitinase ABC in 1 ml of 50 mM Tris-HCl pH 8.0, 60 mM sodium acetate, and 0.02% BSA at 37°C.
Heparitinase, chondroitinase ABC and mAb 3G10 were from Seikagaku.
Heparitinase and chondroitinase ABC treatment of the 35SO 4 labeled samples revealed that the 450 kDa proteoglycan was composed mostly of HSPGs (Fig.
Heparin purified from bovine intestinal mucosa, chondroitin sulfates A, B and C, chondroitinase ABC, sodium chlorate, and Toluidine Blue-O were purchased from Sigma.
Heparitinase treatment also inhibited cell adhesion by 25-40%, while chondroitinase ABC treatment did not inhibit cell adhesion to III1-C (not shown).