Words similar to chondroitinase

Example sentences for: chondroitinase

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Similar assays were followed with chondroitinase ABC, but its substrates, ΔDi4S, dermatan and ΔDi6S, in CM were below detection limits.
Heparitinase treatment also inhibited cell adhesion by 25-40%, while chondroitinase ABC treatment did not inhibit cell adhesion to III1-C (not shown).
For GAGase treatment of intact cells, the cells remained in suspension and received either no enzyme or 0.1 u/ml heparitinase or 0.1 u/ml chondroitinase ABC for 1 hr at 37°C with constant gentle rotation.
Heparitinase and chondroitinase ABC treatment of the 35SO 4 labeled samples revealed that the 450 kDa proteoglycan was composed mostly of HSPGs (Fig.
The soluble material was separated by centrifugation (3000 × g , 10 min) and assayed for absorbance at 235 nm to measure the concentration of product Δ 4-hexuronate (Molar extinction coefficient = 5500; [ 33 ] ). For the chondroitinase ABC reactions, each chondroitin sulfates A, B and C substrate (1 mg each) and concentrated CM, were incubated with 0.12 units of chondroitinase ABC in 1 ml of 50 mM Tris-HCl pH 8.0, 60 mM sodium acetate, and 0.02% BSA at 37°C.