Words similar to chondroitinase

Example sentences for: chondroitinase

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The soluble material was separated by centrifugation (3000 × g , 10 min) and assayed for absorbance at 235 nm to measure the concentration of product Δ 4-hexuronate (Molar extinction coefficient = 5500; [ 33 ] ). For the chondroitinase ABC reactions, each chondroitin sulfates A, B and C substrate (1 mg each) and concentrated CM, were incubated with 0.12 units of chondroitinase ABC in 1 ml of 50 mM Tris-HCl pH 8.0, 60 mM sodium acetate, and 0.02% BSA at 37°C.
Heparitinase, chondroitinase ABC and mAb 3G10 were from Seikagaku.
Heparitinase treatment also inhibited cell adhesion by 25-40%, while chondroitinase ABC treatment did not inhibit cell adhesion to III1-C (not shown).
Similar assays were followed with chondroitinase ABC, but its substrates, ΔDi4S, dermatan and ΔDi6S, in CM were below detection limits.
For GAGase treatment of intact cells, the cells remained in suspension and received either no enzyme or 0.1 u/ml heparitinase or 0.1 u/ml chondroitinase ABC for 1 hr at 37°C with constant gentle rotation.