Example sentences for: cen

How can you use “cen” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • SAT-pADH1-CEN6-ARSH4-HIS3-URA3-SAT

  • Each strain was transformed with a plasmid harboring the respective wild type gene in pRS316 (pSW1268 or pSW1269; see below) [ 66 ] and sporulated to isolate haploid secΔ deletion strains with the p SEC/URA3/CEN plasmid.

  • Strains lacking the wild type SEC/URA3/CEN plasmids were isolated by growth on media containing 5-fluoroorotic acid (5-FOA).

  • In a parallel analysis of the wild type strain (CEN.PK2), dissection yielded 9 tetrads with 4 viable spores, 1 tetrad with 3 viable spores and 1 tetrad with 2 viable spores: average germination 93%.

  • As in the first approach, the TAR vector contained a yeast centromere ( CEN6 ), a yeast ARS element ( ARSH4 ), a positive-selectable marker ( HIS3 ), and a negative-selectable marker ( URA3 ). In this approach, however, the targeting hooks are placed between the promoter and the open reading frame of the URA3 gene.


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