Words similar to cen
Example sentences for: cen
How can you use “cen” in a sentence? Here are some example sentences to help you improve your vocabulary:
3, 22cen, 15q26, 5q33.
All those vectors were created with the Bluescript-based yeast- E. coli shuttle vector pRS313 (HIS3-CEN6-ARSH4) [ 32 ] . The cassette containing the URA3 gene driven by the Shizosaccharomyces pombe ADH1 promoter was derived from the pBL94 plasmid [ 19 ] . In the original cassette, the ADH1 promoter and URA3 were separated by a 35-bp adaptor containing an out-of-frame ATG initiation codon.
The SWY2324, SWY2325, SWY2332, and SWY2333 strains were transformed with a LEU2/CEN yeast genomic library (ATCC, Manassas, VA).
This strategy is similar to one widely used for gene disruption in mice [ 17 ] and was recently successful in isolating chromosomal fragments from a prokaryotic genome [ 18 ] . To apply the strategy, we constructed a TAR vector which, when linearized, had the structure URA3-Hook1-CEN6-ARSH4-HIS3-Hook2 . Hook1 and Hook2 represent two targeting sequences, while URA3 is a marker that can be selected for or against in yeast.
Horizontal arrowheads with "Cen" and "Tel" indicate the orientation of the centromere and q-telomere respectively.