Example sentences for: cen

How can you use “cen” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • In a parallel analysis of the wild type strain (CEN.PK2), dissection yielded 9 tetrads with 4 viable spores, 1 tetrad with 3 viable spores and 1 tetrad with 2 viable spores: average germination 93%.

  • 3, 22cen, 15q26, 5q33.

  • To test the function of the isolated mutant alleles, the resulting haploid secΔ pSEC/URA3/CEN strains were transformed with the mutant sec alleles in a LEU2/CEN plasmid (pSW1264 or pSW1266; see below).

  • Each strain was transformed with a plasmid harboring the respective wild type gene in pRS316 (pSW1268 or pSW1269; see below) [ 66 ] and sporulated to isolate haploid secΔ deletion strains with the p SEC/URA3/CEN plasmid.

  • This strategy is similar to one widely used for gene disruption in mice [ 17 ] and was recently successful in isolating chromosomal fragments from a prokaryotic genome [ 18 ] . To apply the strategy, we constructed a TAR vector which, when linearized, had the structure URA3-Hook1-CEN6-ARSH4-HIS3-Hook2 . Hook1 and Hook2 represent two targeting sequences, while URA3 is a marker that can be selected for or against in yeast.


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