Words similar to cen

Example sentences for: cen

How can you use “cen” in a sentence? Here are some example sentences to help you improve your vocabulary:

It is well documented, however, that linearized ARS/CEN vectors circularize with high efficiency via illegitimate end-joining during yeast transformation [ [ 16 ] and references therein].
This strategy is similar to one widely used for gene disruption in mice [ 17 ] and was recently successful in isolating chromosomal fragments from a prokaryotic genome [ 18 ] . To apply the strategy, we constructed a TAR vector which, when linearized, had the structure URA3-Hook1-CEN6-ARSH4-HIS3-Hook2 . Hook1 and Hook2 represent two targeting sequences, while URA3 is a marker that can be selected for or against in yeast.
All those vectors were created with the Bluescript-based yeast- E. coli shuttle vector pRS313 (HIS3-CEN6-ARSH4) [ 32 ] . The cassette containing the URA3 gene driven by the Shizosaccharomyces pombe ADH1 promoter was derived from the pBL94 plasmid [ 19 ] . In the original cassette, the ADH1 promoter and URA3 were separated by a 35-bp adaptor containing an out-of-frame ATG initiation codon.
As in the first approach, the TAR vector contained a yeast centromere ( CEN6 ), a yeast ARS element ( ARSH4 ), a positive-selectable marker ( HIS3 ), and a negative-selectable marker ( URA3 ). In this approach, however, the targeting hooks are placed between the promoter and the open reading frame of the URA3 gene.
Strains lacking the wild type SEC/URA3/CEN plasmids were isolated by growth on media containing 5-fluoroorotic acid (5-FOA).