Example sentences for: cen

How can you use “cen” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Strains lacking the wild type SEC/URA3/CEN plasmids were isolated by growth on media containing 5-fluoroorotic acid (5-FOA).

  • Each strain was transformed with a plasmid harboring the respective wild type gene in pRS316 (pSW1268 or pSW1269; see below) [ 66 ] and sporulated to isolate haploid secΔ deletion strains with the p SEC/URA3/CEN plasmid.

  • This strategy is similar to one widely used for gene disruption in mice [ 17 ] and was recently successful in isolating chromosomal fragments from a prokaryotic genome [ 18 ] . To apply the strategy, we constructed a TAR vector which, when linearized, had the structure URA3-Hook1-CEN6-ARSH4-HIS3-Hook2 . Hook1 and Hook2 represent two targeting sequences, while URA3 is a marker that can be selected for or against in yeast.

  • Horizontal arrowheads with "Cen" and "Tel" indicate the orientation of the centromere and q-telomere respectively.

  • SAT-pADH1-CEN6-ARSH4-HIS3-URA3-SAT


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