Words similar to cen
Example sentences for: cen
How can you use “cen” in a sentence? Here are some example sentences to help you improve your vocabulary:
SAT-pADH1-CEN6-ARSH4-HIS3-URA3-SAT
Each strain was transformed with a plasmid harboring the respective wild type gene in pRS316 (pSW1268 or pSW1269; see below) [ 66 ] and sporulated to isolate haploid secΔ deletion strains with the p SEC/URA3/CEN plasmid.
Strains lacking the wild type SEC/URA3/CEN plasmids were isolated by growth on media containing 5-fluoroorotic acid (5-FOA).
In a parallel analysis of the wild type strain (CEN.PK2), dissection yielded 9 tetrads with 4 viable spores, 1 tetrad with 3 viable spores and 1 tetrad with 2 viable spores: average germination 93%.
As in the first approach, the TAR vector contained a yeast centromere ( CEN6 ), a yeast ARS element ( ARSH4 ), a positive-selectable marker ( HIS3 ), and a negative-selectable marker ( URA3 ). In this approach, however, the targeting hooks are placed between the promoter and the open reading frame of the URA3 gene.