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Example sentences for: cathd

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cathD PCR
Appropriate pairs of gene and element primers were used to detect the presence or absence of the respective retrotransposon inserts associated with each gene in strains representing 12 geographically dispersed populations of D. melanogaster . The results presented in Figure 2and Table 1demonstrate that while the Burdock insertion located just 3' to cathD gene is not present in any of 12 strains representing a geographically diverse sampling of natural populations, the Antonia LTR fragment located in the intron of the heterochromatic Cht3 gene is fixed in all 12 strains tested.
Genomic analysis of the sequenced y; cn bw sp strain of Drosophila melanogaster identified a full-length Burdock LTR retrotransposon located just 3' to the cathD gene and a 359bp LTR fragment (complete LTR is 659 bp) of an Antonia LTR retrotransposon [ 15 ] located within an intron of the Cht3 gene (Figure 1).
Our findings demonstrate that while the euchromatic cathD insertion variant was not detected in any of the natural populations examined, the insertion variant present in the heterochromatic Cht3 gene was found to be apparently fixed throughout the species.
The annealing temperature for primer set cathD(f) and cathD(r) is 58°C, for primer set Burdock LTR(f) and Burdock element(r) is 59°C, and for primer set cathDff ) and Burdock element(r) is 56°C.